Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- V3517 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Prohibitin 1 Antibody / PHB1
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human, Other
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- PHB/1881
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml; 1 mg/ml (BSA/azide-free format)
- Storage
- Store the Prohibitin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HEK293, 2) Jurkat, 3) HepG2 and 4) U-87 MG cell lysate with Prohibitin antibody (clone PHB/1881). Expected molecular weight ~30 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human cell lysates with Prohibitin antibody (clone PHB/1881). Expected molecular weight ~30 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver tissue with Prohibitin antibody (clone PHB/1881). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Prohibitin Antibody (clone PHB/1881). Confirmation of Integrity and Purity of the Antibody.
