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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-87283 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87283, RRID:AB_11005625
- Product name
- Rabbit Polyclonal Prohibitin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat Prohibitin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The unique stem cell system of the immortal larva of the human parasite Echinococcus multilocularis.
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Koziol U, Rauschendorfer T, Zanon Rodríguez L, Krohne G, Brehm K
EvoDevo 2014 Mar 6;5(1):10
EvoDevo 2014 Mar 6;5(1):10
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Prohibitin Antibody [NBP1-87283] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Prohibitin Antibody [NBP1-87283] - Analysis in human cell lines Caco-2 and HeLa using Anti-PHB antibody. Corresponding PHB RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP1-87283] - Staining of human stomach shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.
