MA5-12855

antibody from Invitrogen Antibodies

Targeting: PHB PHB1

Antibody data

Submit

Validation data

Product number: MA5-12855 - Provider product page
Provider: Invitrogen Antibodies
Product name: Prohibitin Monoclonal Antibody (II-14-10), Biotin
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-12855 targets Prohibitin in IF, IHC (P), and WB applications and shows reactivity with Chicken, Human, mouse, Porcine, and Rat samples. The MA5-12855 immunogen is purified recombinant rat prohibitin protein.
Reactivity: Human, Mouse, Rat, Chicken/Avian, Porcine
Host: Mouse
Conjugate: Biotin
Isotype: IgG
Antibody clone number: II-14-10
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

PHB protein structure - MA5-12855 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of Prohibitin using Prohibitin Monoclonal Antibody (Product # MA5-12855) on PC12 Cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Prohibitin was achieved by transfecting HEPG2 cells with Prohibitin specific siRNAs (Silencer® select Product # s10424 and s10425). Western blot analysis (Fig a) was performed using Membrane extract from the Prohibitin knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Prohibitin biotin Mouse Monoclonal Antibody (Product # MA5-12855, 0.2 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to Prohibitin.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg of lysate) of Caco-2 (Lane 1), Caco-2 treated with H2O2 (100uM for 1 hr) (Lane2), Neuro-2a (Lane 3), Neuro-2a treated with H2O2 (100uM for 1 hr) (Lane 4), COS-7 (Lane 5), COS-7 treated with H2O2 (100uM for 1 hr) (Lane 6) and HepG2 (Lane 7). The blots were probed with anti-Prohibitin Mouse Monoclonal Antibody, Biotin (Product # MA5-12855, 1 µg/mL) and detected by chemiluminescence. A 30 kDa band corresponding to Prohibitin was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg of lysate) of Caco-2 (Lane 1), Caco-2 treated with H2O2 (100uM for 1 hr) (Lane2), Neuro-2a (Lane 3), Neuro-2a treated with H2O2 (100uM for 1 hr) (Lane 4), COS-7 (Lane 5), COS-7 treated with H2O2 (100uM for 1 hr) (Lane 6) and HepG2 (Lane 7). The blots were probed with anti-Prohibitin Mouse Monoclonal Antibody, Biotin (Product # MA5-12855, 1 µg/mL) and detected by chemiluminescence. A 30 kDa band corresponding to Prohibitin was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Conjugate: Biotin