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- Antigen structure
- References [68]
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- Product number
- MA5-12858 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Prohibitin Monoclonal Antibody (II-14-10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-12858 targets Prohibitin in immunofluorescence, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with Chicken, Human, mouse, Porcine, and Rat samples. The MA5-12858 immunogen is purified recombinant rat prohibitin protein.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- II-14-10
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references A novel function of Prohibitin on melanosome transport in melanocytes.
Prohibitin (PHB) interacts with AKT in mitochondria to coordinately modulate sperm motility.
Prohibitin levels regulate OMA1 activity and turnover in neurons.
Stop codon read-through of mammalian MTCH2 leading to an unstable isoform regulates mitochondrial membrane potential.
Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales.
The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.
Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours.
Transglutaminase type 2-dependent selective recruitment of proteins into exosomes under stressful cellular conditions.
Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
Quantitative proteomics identifies host factors modulated during acute hepatitis E virus infection in the swine model.
Prohibitin expression deregulation in gastric cancer is associated with the 3' untranslated region 1630 C>T polymorphism and copy number variation.
Prohibitin is involved in the activated internalization and degradation of protease-activated receptor 1.
Interaction of delta-like 1 homolog (Drosophila) with prohibitins and its impact on tumor cell clonogenicity.
Differential expression and subcellular localization of Prohibitin 1 are related to tumorigenesis and progression of non-small cell lung cancer.
Prohibitins function as endogenous ligands for Siglec-9 and negatively regulate TCR signaling upon ligation.
GRSF1 regulates RNA processing in mitochondrial RNA granules.
Proteomic, cellular, and network analyses reveal new DUSP3 interactions with nucleolar proteins in HeLa cells.
Nuclear accumulation of prohibitin 1 in osteoarthritic chondrocytes down-regulates PITX1 expression.
General Sensitization of melanoma cells for TRAIL-induced apoptosis by the potassium channel inhibitor TRAM-34 depends on release of SMAC.
Induction of apoptosis in macrophages via Kv1.3 and Kv1.5 potassium channels.
Induction of apoptosis in macrophages via Kv1.3 and Kv1.5 potassium channels.
All-trans retinoic acid treatment is associated with prohibitin expression in renal interstitial fibrosis rats.
Induction of the permeability transition pore in cells depleted of mitochondrial DNA.
Reducing prohibitin increases histone acetylation, and promotes androgen independence in prostate tumours by increasing androgen receptor activation by adrenal androgens.
Manganese superoxide dismutase: a regulator of T cell activation-induced oxidative signaling and cell death.
Proteomic and protein interaction network analysis of human T lymphocytes during cell-cycle entry.
Inhibitors of mitochondrial Kv1.3 channels induce Bax/Bak-independent death of cancer cells.
Androgen-regulated processing of the oncomir miR-27a, which targets Prohibitin in prostate cancer.
Expression of prohibitins on the surface of activated T cells.
Nanoparticle-based therapeutic delivery of prohibitin to the colonic epithelial cells ameliorates acute murine colitis.
Intrauterine growth restriction and postnatal high-protein diet affect the kidneys in adult rats.
EndoG links Bnip3-induced mitochondrial damage and caspase-independent DNA fragmentation in ischemic cardiomyocytes.
The dynamic equilibrium between ATP synthesis and ATP consumption is lower in isolated mitochondria from myotubes established from type 2 diabetic subjects compared to lean control.
Separation and identification of differentially expressed nuclear matrix proteins in breast carcinoma forming.
Proteomic study of thyroid tumors reveals frequent up-regulation of the Ca2+ -binding protein S100A6 in papillary thyroid carcinoma.
An investigation of the occurrence and properties of the mitochondrial intermediate-conductance Ca2+-activated K+ channel mtKCa3.1.
Proteome analysis of ultraviolet-B-induced protein expression in vitro human dermal fibroblasts.
Identification of new modulators and protein alterations in non-apoptotic programmed cell death.
Prohibitin as an oxidative stress biomarker in the eye.
An unusual function of RON receptor tyrosine kinase as a transcriptional regulator in cooperation with EGFR in human cancer cells.
Leishmania cell surface prohibitin: role in host-parasite interaction.
Prohibitin inhibits tumor necrosis factor alpha-induced nuclear factor-kappa B nuclear translocation via the novel mechanism of decreasing importin alpha3 expression.
Parkin protects mitochondrial genome integrity and supports mitochondrial DNA repair.
Nuclear matrix protein, prohibitin, was down-regulated and translocated from nucleus to cytoplasm during the differentiation of osteosarcoma MG-63 cells induced by ginsenoside Rg1, cinnamic acid, and tanshinone IIA (RCT).
Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells.
Spontaneous internalization of IVIg in activated B cells.
Overexpression of follicle-stimulating hormone receptor facilitates the development of ovarian epithelial cancer.
Cellular vimentin content regulates the protein level of hepatitis C virus core protein and the hepatitis C virus production in cultured cells.
Requirement for chromatin-remodeling complex in novel tumor suppressor HIC1-mediated transcriptional repression and growth control.
Contribution of nitric oxide, superoxide anion, and peroxynitrite to activation of mitochondrial apoptotic signaling in hippocampal CA3 subfield following experimental temporal lobe status epilepticus.
Purification and proteomic analysis of the mouse liver mitochondrial inner membrane.
Development of hepatocellular carcinoma in Iqgap2-deficient mice is IQGAP1 dependent.
Structural analysis of tissues affected by cytochrome C oxidase deficiency due to mutations in the SCO2 gene.
Development of the terminally differentiated state sensitizes epiphyseal chondrocytes to apoptosis through caspase-3 activation.
Prohibitin in the pathogenesis of transitional cell bladder cancer.
A case of excessive autophagocytosis with multiorgan involvement and low clinical penetrance.
Prohibitin suppresses renal interstitial fibroblasts proliferation and phenotypic change induced by transforming growth factor-beta1.
Differential regulation of human YY1 and caspase 7 promoters by prohibitin through E2F1 and p53 binding sites.
Neurolysosomal pathology in human prosaposin deficiency suggests essential neurotrophic function of prosaposin.
Heat shock protein 60 or 70 activates nitric-oxide synthase (NOS) I- and inhibits NOS II-associated signaling and depresses the mitochondrial apoptotic cascade during brain stem death.
Upregulation of nitric oxide synthase II contributes to apoptotic cell death in the hippocampal CA3 subfield via a cytochrome c/caspase-3 signaling cascade following induction of experimental temporal lobe status epilepticus in the rat.
Heat shock protein 60 in rostral ventrolateral medulla reduces cardiovascular fatality during endotoxaemia in the rat.
Differential regulation of metabolic genes in skeletal muscle during starvation and refeeding in humans.
Proteomic analysis of redox- and ErbB2-dependent changes in mammary luminal epithelial cells using cysteine- and lysine-labelling two-dimensional difference gel electrophoresis.
Molecular determinants for subcellular localization of hepatitis C virus core protein.
Protein expression profiles in adenocarcinomas and squamous cell carcinomas of the lung generated using tissue microarrays.
A death receptor-associated anti-apoptotic protein, BRE, inhibits mitochondrial apoptotic pathway.
Ubiquitination of prohibitin in mammalian sperm mitochondria: possible roles in the regulation of mitochondrial inheritance and sperm quality control.
Jo CS, Park HI, Jung HJ, Park JI, Lee JE, Myung CH, Hwang JS
Theranostics 2020;10(9):3880-3891
Theranostics 2020;10(9):3880-3891
Prohibitin (PHB) interacts with AKT in mitochondria to coordinately modulate sperm motility.
Li XH, Chai RR, Chen GW, Zhang LF, Tan-Tai WJ, Shi HJ, Martin-DeLeon PA, O WS, Chen H
Asian journal of andrology 2020 Nov-Dec;22(6):583-589
Asian journal of andrology 2020 Nov-Dec;22(6):583-589
Prohibitin levels regulate OMA1 activity and turnover in neurons.
Anderson CJ, Kahl A, Fruitman H, Qian L, Zhou P, Manfredi G, Iadecola C
Cell death and differentiation 2020 Jun;27(6):1896-1906
Cell death and differentiation 2020 Jun;27(6):1896-1906
Stop codon read-through of mammalian MTCH2 leading to an unstable isoform regulates mitochondrial membrane potential.
Manjunath LE, Singh A, Sahoo S, Mishra A, Padmarajan J, Basavaraju CG, Eswarappa SM
The Journal of biological chemistry 2020 Dec 11;295(50):17009-17026
The Journal of biological chemistry 2020 Dec 11;295(50):17009-17026
Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales.
Ludwig AK, De Miroschedji K, Doeppner TR, Börger V, Ruesing J, Rebmann V, Durst S, Jansen S, Bremer M, Behrmann E, Singer BB, Jastrow H, Kuhlmann JD, El Magraoui F, Meyer HE, Hermann DM, Opalka B, Raunser S, Epple M, Horn PA, Giebel B
Journal of extracellular vesicles 2018;7(1):1528109
Journal of extracellular vesicles 2018;7(1):1528109
The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.
Zaganelli S, Rebelo-Guiomar P, Maundrell K, Rozanska A, Pierredon S, Powell CA, Jourdain AA, Hulo N, Lightowlers RN, Chrzanowska-Lightowlers ZM, Minczuk M, Martinou JC
The Journal of biological chemistry 2017 Mar 17;292(11):4519-4532
The Journal of biological chemistry 2017 Mar 17;292(11):4519-4532
Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours.
Yurugi H, Marini F, Weber C, David K, Zhao Q, Binder H, Désaubry L, Rajalingam K
Oncogene 2017 Aug 17;36(33):4778-4789
Oncogene 2017 Aug 17;36(33):4778-4789
Transglutaminase type 2-dependent selective recruitment of proteins into exosomes under stressful cellular conditions.
Diaz-Hidalgo L, Altuntas S, Rossin F, D'Eletto M, Marsella C, Farrace MG, Falasca L, Antonioli M, Fimia GM, Piacentini M
Biochimica et biophysica acta 2016 Aug;1863(8):2084-92
Biochimica et biophysica acta 2016 Aug;1863(8):2084-92
Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
Poitelon Y, Bogni S, Matafora V, Della-Flora Nunes G, Hurley E, Ghidinelli M, Katzenellenbogen BS, Taveggia C, Silvestri N, Bachi A, Sannino A, Wrabetz L, Feltri ML
Nature communications 2015 Sep 18;6:8303
Nature communications 2015 Sep 18;6:8303
Quantitative proteomics identifies host factors modulated during acute hepatitis E virus infection in the swine model.
Rogée S, Le Gall M, Chafey P, Bouquet J, Cordonnier N, Frederici C, Pavio N
Journal of virology 2015 Jan;89(1):129-43
Journal of virology 2015 Jan;89(1):129-43
Prohibitin expression deregulation in gastric cancer is associated with the 3' untranslated region 1630 C>T polymorphism and copy number variation.
Leal MF, Cirilo PD, Mazzotti TK, Calcagno DQ, Wisnieski F, Demachki S, Martinez MC, Assumpção PP, Chammas R, Burbano RR, Smith MC
PloS one 2014;9(5):e98583
PloS one 2014;9(5):e98583
Prohibitin is involved in the activated internalization and degradation of protease-activated receptor 1.
Wang YJ, Guo XL, Li SA, Zhao YQ, Liu ZC, Lee WH, Xiang Y, Zhang Y
Biochimica et biophysica acta 2014 Jul;1843(7):1393-401
Biochimica et biophysica acta 2014 Jul;1843(7):1393-401
Interaction of delta-like 1 homolog (Drosophila) with prohibitins and its impact on tumor cell clonogenicity.
Begum A, Lin Q, Yu C, Kim Y, Yun Z
Molecular cancer research : MCR 2014 Jan;12(1):155-64
Molecular cancer research : MCR 2014 Jan;12(1):155-64
Differential expression and subcellular localization of Prohibitin 1 are related to tumorigenesis and progression of non-small cell lung cancer.
Jiang P, Xiang Y, Wang YJ, Li SM, Wang Y, Hua HR, Yu GY, Zhang Y, Lee WH, Zhang Y
International journal of clinical and experimental pathology 2013;6(10):2092-101
International journal of clinical and experimental pathology 2013;6(10):2092-101
Prohibitins function as endogenous ligands for Siglec-9 and negatively regulate TCR signaling upon ligation.
Yurugi H, Tanida S, Akita K, Ishida A, Toda M, Nakada H
Biochemical and biophysical research communications 2013 May 3;434(2):376-81
Biochemical and biophysical research communications 2013 May 3;434(2):376-81
GRSF1 regulates RNA processing in mitochondrial RNA granules.
Jourdain AA, Koppen M, Wydro M, Rodley CD, Lightowlers RN, Chrzanowska-Lightowlers ZM, Martinou JC
Cell metabolism 2013 Mar 5;17(3):399-410
Cell metabolism 2013 Mar 5;17(3):399-410
Proteomic, cellular, and network analyses reveal new DUSP3 interactions with nucleolar proteins in HeLa cells.
Panico K, Forti FL
Journal of proteome research 2013 Dec 6;12(12):5851-66
Journal of proteome research 2013 Dec 6;12(12):5851-66
Nuclear accumulation of prohibitin 1 in osteoarthritic chondrocytes down-regulates PITX1 expression.
Picard C, Pellicelli M, Taheri M, Lavoie JF, Doucet R, Wang D, Bernard L, Bouhanik S, Lavigne P, Moreau A
Arthritis and rheumatism 2013 Apr;65(4):993-1003
Arthritis and rheumatism 2013 Apr;65(4):993-1003
General Sensitization of melanoma cells for TRAIL-induced apoptosis by the potassium channel inhibitor TRAM-34 depends on release of SMAC.
Quast SA, Berger A, Buttstädt N, Friebel K, Schönherr R, Eberle J
PloS one 2012;7(6):e39290
PloS one 2012;7(6):e39290
Induction of apoptosis in macrophages via Kv1.3 and Kv1.5 potassium channels.
Leanza L, Zoratti M, Gulbins E, Szabò I
Current medicinal chemistry 2012;19(31):5394-404
Current medicinal chemistry 2012;19(31):5394-404
Induction of apoptosis in macrophages via Kv1.3 and Kv1.5 potassium channels.
Leanza L, Zoratti M, Gulbins E, Szabò I
Current medicinal chemistry 2012;19(31):5394-404
Current medicinal chemistry 2012;19(31):5394-404
All-trans retinoic acid treatment is associated with prohibitin expression in renal interstitial fibrosis rats.
Zhou TB, Qin YH, Li ZY, Xu HL, Zhao YJ, Lei FY
International journal of molecular sciences 2012;13(3):2769-2782
International journal of molecular sciences 2012;13(3):2769-2782
Induction of the permeability transition pore in cells depleted of mitochondrial DNA.
Masgras I, Rasola A, Bernardi P
Biochimica et biophysica acta 2012 Oct;1817(10):1860-6
Biochimica et biophysica acta 2012 Oct;1817(10):1860-6
Reducing prohibitin increases histone acetylation, and promotes androgen independence in prostate tumours by increasing androgen receptor activation by adrenal androgens.
Dart DA, Brooke GN, Sita-Lumsden A, Waxman J, Bevan CL
Oncogene 2012 Oct 25;31(43):4588-98
Oncogene 2012 Oct 25;31(43):4588-98
Manganese superoxide dismutase: a regulator of T cell activation-induced oxidative signaling and cell death.
Kamiński MM, Röth D, Sass S, Sauer SW, Krammer PH, Gülow K
Biochimica et biophysica acta 2012 May;1823(5):1041-52
Biochimica et biophysica acta 2012 May;1823(5):1041-52
Proteomic and protein interaction network analysis of human T lymphocytes during cell-cycle entry.
Orr SJ, Boutz DR, Wang R, Chronis C, Lea NC, Thayaparan T, Hamilton E, Milewicz H, Blanc E, Mufti GJ, Marcotte EM, Thomas NS
Molecular systems biology 2012 Mar 13;8:573
Molecular systems biology 2012 Mar 13;8:573
Inhibitors of mitochondrial Kv1.3 channels induce Bax/Bak-independent death of cancer cells.
Leanza L, Henry B, Sassi N, Zoratti M, Chandy KG, Gulbins E, Szabò I
EMBO molecular medicine 2012 Jul;4(7):577-93
EMBO molecular medicine 2012 Jul;4(7):577-93
Androgen-regulated processing of the oncomir miR-27a, which targets Prohibitin in prostate cancer.
Fletcher CE, Dart DA, Sita-Lumsden A, Cheng H, Rennie PS, Bevan CL
Human molecular genetics 2012 Jul 15;21(14):3112-27
Human molecular genetics 2012 Jul 15;21(14):3112-27
Expression of prohibitins on the surface of activated T cells.
Yurugi H, Tanida S, Ishida A, Akita K, Toda M, Inoue M, Nakada H
Biochemical and biophysical research communications 2012 Apr 6;420(2):275-80
Biochemical and biophysical research communications 2012 Apr 6;420(2):275-80
Nanoparticle-based therapeutic delivery of prohibitin to the colonic epithelial cells ameliorates acute murine colitis.
Theiss AL, Laroui H, Obertone TS, Chowdhury I, Thompson WE, Merlin D, Sitaraman SV
Inflammatory bowel diseases 2011 May;17(5):1163-76
Inflammatory bowel diseases 2011 May;17(5):1163-76
Intrauterine growth restriction and postnatal high-protein diet affect the kidneys in adult rats.
Shen Q, Xu H, Wei LM, Chen J, Liu HM
Nutrition (Burbank, Los Angeles County, Calif.) 2011 Mar;27(3):364-71
Nutrition (Burbank, Los Angeles County, Calif.) 2011 Mar;27(3):364-71
EndoG links Bnip3-induced mitochondrial damage and caspase-independent DNA fragmentation in ischemic cardiomyocytes.
Zhang J, Ye J, Altafaj A, Cardona M, Bahi N, Llovera M, Cañas X, Cook SA, Comella JX, Sanchis D
PloS one 2011 Mar 17;6(3):e17998
PloS one 2011 Mar 17;6(3):e17998
The dynamic equilibrium between ATP synthesis and ATP consumption is lower in isolated mitochondria from myotubes established from type 2 diabetic subjects compared to lean control.
Minet AD, Gaster M
Biochemical and biophysical research communications 2011 Jun 17;409(4):591-5
Biochemical and biophysical research communications 2011 Jun 17;409(4):591-5
Separation and identification of differentially expressed nuclear matrix proteins in breast carcinoma forming.
He Q, Zhang SQ, Chu YL, Jia XL, Zhao LH, Wang XL
Acta oncologica (Stockholm, Sweden) 2010;49(1):76-84
Acta oncologica (Stockholm, Sweden) 2010;49(1):76-84
Proteomic study of thyroid tumors reveals frequent up-regulation of the Ca2+ -binding protein S100A6 in papillary thyroid carcinoma.
Sofiadis A, Dinets A, Orre LM, Branca RM, Juhlin CC, Foukakis T, Wallin G, Höög A, Hulchiy M, Zedenius J, Larsson C, Lehtiö J
Thyroid : official journal of the American Thyroid Association 2010 Oct;20(10):1067-76
Thyroid : official journal of the American Thyroid Association 2010 Oct;20(10):1067-76
An investigation of the occurrence and properties of the mitochondrial intermediate-conductance Ca2+-activated K+ channel mtKCa3.1.
Sassi N, De Marchi U, Fioretti B, Biasutto L, Gulbins E, Franciolini F, Szabò I, Zoratti M
Biochimica et biophysica acta 2010 Jun-Jul;1797(6-7):1260-7
Biochimica et biophysica acta 2010 Jun-Jul;1797(6-7):1260-7
Proteome analysis of ultraviolet-B-induced protein expression in vitro human dermal fibroblasts.
Yan Y, Xu H, Peng S, Zhao W, Wang B
Photodermatology, photoimmunology & photomedicine 2010 Dec;26(6):318-26
Photodermatology, photoimmunology & photomedicine 2010 Dec;26(6):318-26
Identification of new modulators and protein alterations in non-apoptotic programmed cell death.
Sperandio S, Poksay KS, Schilling B, Crippen D, Gibson BW, Bredesen DE
Journal of cellular biochemistry 2010 Dec 15;111(6):1401-12
Journal of cellular biochemistry 2010 Dec 15;111(6):1401-12
Prohibitin as an oxidative stress biomarker in the eye.
Lee H, Arnouk H, Sripathi S, Chen P, Zhang R, Bartoli M, Hunt RC, Hrushesky WJ, Chung H, Lee SH, Jahng WJ
International journal of biological macromolecules 2010 Dec 1;47(5):685-90
International journal of biological macromolecules 2010 Dec 1;47(5):685-90
An unusual function of RON receptor tyrosine kinase as a transcriptional regulator in cooperation with EGFR in human cancer cells.
Liu HS, Hsu PY, Lai MD, Chang HY, Ho CL, Cheng HL, Chen HT, Lin YJ, Wu TJ, Tzai TS, Chow NH
Carcinogenesis 2010 Aug;31(8):1456-64
Carcinogenesis 2010 Aug;31(8):1456-64
Leishmania cell surface prohibitin: role in host-parasite interaction.
Jain R, Ghoshal A, Mandal C, Shaha C
Cellular microbiology 2010 Apr 1;12(4):432-52
Cellular microbiology 2010 Apr 1;12(4):432-52
Prohibitin inhibits tumor necrosis factor alpha-induced nuclear factor-kappa B nuclear translocation via the novel mechanism of decreasing importin alpha3 expression.
Theiss AL, Jenkins AK, Okoro NI, Klapproth JM, Merlin D, Sitaraman SV
Molecular biology of the cell 2009 Oct;20(20):4412-23
Molecular biology of the cell 2009 Oct;20(20):4412-23
Parkin protects mitochondrial genome integrity and supports mitochondrial DNA repair.
Rothfuss O, Fischer H, Hasegawa T, Maisel M, Leitner P, Miesel F, Sharma M, Bornemann A, Berg D, Gasser T, Patenge N
Human molecular genetics 2009 Oct 15;18(20):3832-50
Human molecular genetics 2009 Oct 15;18(20):3832-50
Nuclear matrix protein, prohibitin, was down-regulated and translocated from nucleus to cytoplasm during the differentiation of osteosarcoma MG-63 cells induced by ginsenoside Rg1, cinnamic acid, and tanshinone IIA (RCT).
Shi SL, Li QF, Liu QR, Xu DH, Tang J, Liang Y, Zhao ZL, Yang LM
Journal of cellular biochemistry 2009 Nov 1;108(4):926-34
Journal of cellular biochemistry 2009 Nov 1;108(4):926-34
Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells.
De Marchi U, Sassi N, Fioretti B, Catacuzzeno L, Cereghetti GM, Szabò I, Zoratti M
Cell calcium 2009 May;45(5):509-16
Cell calcium 2009 May;45(5):509-16
Spontaneous internalization of IVIg in activated B cells.
Proulx DP, Aubin E, Lemieux R, Bazin R
Immunology letters 2009 May 14;124(1):18-26
Immunology letters 2009 May 14;124(1):18-26
Overexpression of follicle-stimulating hormone receptor facilitates the development of ovarian epithelial cancer.
Zhang Z, Jia L, Feng Y, Zheng W
Cancer letters 2009 Jun 8;278(1):56-64
Cancer letters 2009 Jun 8;278(1):56-64
Cellular vimentin content regulates the protein level of hepatitis C virus core protein and the hepatitis C virus production in cultured cells.
Nitahara-Kasahara Y, Fukasawa M, Shinkai-Ouchi F, Sato S, Suzuki T, Murakami K, Wakita T, Hanada K, Miyamura T, Nishijima M
Virology 2009 Jan 20;383(2):319-27
Virology 2009 Jan 20;383(2):319-27
Requirement for chromatin-remodeling complex in novel tumor suppressor HIC1-mediated transcriptional repression and growth control.
Zhang B, Chambers KJ, Leprince D, Faller DV, Wang S
Oncogene 2009 Feb 5;28(5):651-61
Oncogene 2009 Feb 5;28(5):651-61
Contribution of nitric oxide, superoxide anion, and peroxynitrite to activation of mitochondrial apoptotic signaling in hippocampal CA3 subfield following experimental temporal lobe status epilepticus.
Chuang YC, Chen SD, Liou CW, Lin TK, Chang WN, Chan SH, Chang AY
Epilepsia 2009 Apr;50(4):731-46
Epilepsia 2009 Apr;50(4):731-46
Purification and proteomic analysis of the mouse liver mitochondrial inner membrane.
Da Cruz S, Martinou JC
Methods in molecular biology (Clifton, N.J.) 2008;432:101-16
Methods in molecular biology (Clifton, N.J.) 2008;432:101-16
Development of hepatocellular carcinoma in Iqgap2-deficient mice is IQGAP1 dependent.
Schmidt VA, Chiariello CS, Capilla E, Miller F, Bahou WF
Molecular and cellular biology 2008 Mar;28(5):1489-502
Molecular and cellular biology 2008 Mar;28(5):1489-502
Structural analysis of tissues affected by cytochrome C oxidase deficiency due to mutations in the SCO2 gene.
Vesela K, Hulkova H, Hansikova H, Zeman J, Elleder M
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2008 Jan;116(1):41-9
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2008 Jan;116(1):41-9
Development of the terminally differentiated state sensitizes epiphyseal chondrocytes to apoptosis through caspase-3 activation.
Pucci B, Adams CS, Fertala J, Snyder BC, Mansfield KD, Tafani M, Freeman T, Shapiro IM
Journal of cellular physiology 2007 Mar;210(3):609-15
Journal of cellular physiology 2007 Mar;210(3):609-15
Prohibitin in the pathogenesis of transitional cell bladder cancer.
Wu TF, Wu H, Wang YW, Chang TY, Chan SH, Lin YP, Liu HS, Chow NH
Anticancer research 2007 Mar-Apr;27(2):895-900
Anticancer research 2007 Mar-Apr;27(2):895-900
A case of excessive autophagocytosis with multiorgan involvement and low clinical penetrance.
Sikora J, Dvoráková L, Vlásková H, Stolnaja L, Betlach J, Spacek J, Elleder M
Ceskoslovenska patologie 2007 Jul;43(3):93-103
Ceskoslovenska patologie 2007 Jul;43(3):93-103
Prohibitin suppresses renal interstitial fibroblasts proliferation and phenotypic change induced by transforming growth factor-beta1.
Guo W, Xu H, Chen J, Yang Y, Jin JW, Fu R, Liu HM, Zha XL, Zhang ZG, Huang WY
Molecular and cellular biochemistry 2007 Jan;295(1-2):167-77
Molecular and cellular biochemistry 2007 Jan;295(1-2):167-77
Differential regulation of human YY1 and caspase 7 promoters by prohibitin through E2F1 and p53 binding sites.
Joshi B, Rastogi S, Morris M, Carastro LM, DeCook C, Seto E, Chellappan SP
The Biochemical journal 2007 Jan 1;401(1):155-66
The Biochemical journal 2007 Jan 1;401(1):155-66
Neurolysosomal pathology in human prosaposin deficiency suggests essential neurotrophic function of prosaposin.
Sikora J, Harzer K, Elleder M
Acta neuropathologica 2007 Feb;113(2):163-75
Acta neuropathologica 2007 Feb;113(2):163-75
Heat shock protein 60 or 70 activates nitric-oxide synthase (NOS) I- and inhibits NOS II-associated signaling and depresses the mitochondrial apoptotic cascade during brain stem death.
Chan JYH, Cheng HL, Chou JLJ, Li FCH, Dai KY, Chan SHH, Chang AYW
The Journal of biological chemistry 2007 Feb 16;282(7):4585-4600
The Journal of biological chemistry 2007 Feb 16;282(7):4585-4600
Upregulation of nitric oxide synthase II contributes to apoptotic cell death in the hippocampal CA3 subfield via a cytochrome c/caspase-3 signaling cascade following induction of experimental temporal lobe status epilepticus in the rat.
Chuang YC, Chen SD, Lin TK, Liou CW, Chang WN, Chan SH, Chang AY
Neuropharmacology 2007 Apr;52(5):1263-73
Neuropharmacology 2007 Apr;52(5):1263-73
Heat shock protein 60 in rostral ventrolateral medulla reduces cardiovascular fatality during endotoxaemia in the rat.
Chang AY, Chan JY, Chou JL, Li FC, Dai KY, Chan SH
The Journal of physiology 2006 Jul 15;574(Pt 2):547-64
The Journal of physiology 2006 Jul 15;574(Pt 2):547-64
Differential regulation of metabolic genes in skeletal muscle during starvation and refeeding in humans.
Tsintzas K, Jewell K, Kamran M, Laithwaite D, Boonsong T, Littlewood J, Macdonald I, Bennett A
The Journal of physiology 2006 Aug 15;575(Pt 1):291-303
The Journal of physiology 2006 Aug 15;575(Pt 1):291-303
Proteomic analysis of redox- and ErbB2-dependent changes in mammary luminal epithelial cells using cysteine- and lysine-labelling two-dimensional difference gel electrophoresis.
Chan HL, Gharbi S, Gaffney PR, Cramer R, Waterfield MD, Timms JF
Proteomics 2005 Jul;5(11):2908-26
Proteomics 2005 Jul;5(11):2908-26
Molecular determinants for subcellular localization of hepatitis C virus core protein.
Suzuki R, Sakamoto S, Tsutsumi T, Rikimaru A, Tanaka K, Shimoike T, Moriishi K, Iwasaki T, Mizumoto K, Matsuura Y, Miyamura T, Suzuki T
Journal of virology 2005 Jan;79(2):1271-81
Journal of virology 2005 Jan;79(2):1271-81
Protein expression profiles in adenocarcinomas and squamous cell carcinomas of the lung generated using tissue microarrays.
Ullmann R, Morbini P, Halbwedl I, Bongiovanni M, Gogg-Kammerer M, Papotti M, Gabor S, Renner H, Popper HH
The Journal of pathology 2004 Jul;203(3):798-807
The Journal of pathology 2004 Jul;203(3):798-807
A death receptor-associated anti-apoptotic protein, BRE, inhibits mitochondrial apoptotic pathway.
Li Q, Ching AK, Chan BC, Chow SK, Lim PL, Ho TC, Ip WK, Wong CK, Lam CW, Lee KK, Chan JY, Chui YL
The Journal of biological chemistry 2004 Dec 10;279(50):52106-16
The Journal of biological chemistry 2004 Dec 10;279(50):52106-16
Ubiquitination of prohibitin in mammalian sperm mitochondria: possible roles in the regulation of mitochondrial inheritance and sperm quality control.
Thompson WE, Ramalho-Santos J, Sutovsky P
Biology of reproduction 2003 Jul;69(1):254-60
Biology of reproduction 2003 Jul;69(1):254-60
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Supportive validation
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- Immunoprecipitation of Prohibitin using Prohibitin Monoclonal Antibody (Product # MA5-12858) on denatured Mouse MAD109 Cells.
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- Immunoprecipitation of Prohibitin using Prohibitin Monoclonal Antibody (Product # MA5-12858) on Native Mouse MAD109 Cells.
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- FIGURE 2. Submitochondrial localization of the mitochondrial pseudouridine synthases. A , confocal analysis of each of the five mitochondrial pseudouridine synthases. 143B cells were transfected with expression plasmids encoding the FLAG-tagged proteins indicated. Mitochondria were stained using MitoTracker Deep Red FM (in blue ). The cells were immunolabeled with anti-FLAG and anti-FASTKD2 as an MRG-specific marker. White boxes indicate the regions shown at higher magnification. Scale bars are 10 mum. B , subcellular fractionation of 143B cells and analysis by Western blotting show enrichment for the endogenous RPUSD4 in the mitochondrial fraction. Total cell lysate ( T ), mitochondrial ( M ), and cytosolic ( C ) fractions were assessed for purity using beta-tubulin and VDAC1 as cytosolic and mitochondrial markers, respectively. C , immunoblotting analysis of total isolated 143B mitochondria ( T ) or the supernatant ( S ) and pellet ( P ) fractions following alkaline sodium carbonate ( Na 2 CO 3 ) extraction. mtHSP70 and prohibitin ( PHB ) are markers for the mitochondrial matrix and mitochondrial membranes, respectively. D , immunoblot analysis of isolated 143B mitochondria after proteinase K ( PK ) accessibility test. Mitochondria were left untreated, swollen to rupture of the outer membrane, or lysed in Triton X-100 prior to treatment with proteinase K. mtHSP70 is a mitochondrial matrix protein, Smac/DIABLO is an intermembrane space protein, and TOM20 is a mitochondrial o
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- Figure 7. Differential localization of MTCH2 isoforms. A, plasmids expressing FLAG-HA-tagged isoforms of MTCH2 were transfected in HEK293 cells. Mitochondrial and cytoplasmic fractions from these cells were used for Western blotting. VDAC and alpha-tubulin were used as markers for mitochondria and cytoplasm, respectively. B, mitochondrial localization of endogenous MTCH2 and MTCH2x in HEK293 cells. Prohibitin was used as a marker for mitochondria. C, confocal fluorescence microscopy images showing cellular localization of GFP-tagged MTCH2 isoforms in primary bovine aortic endothelial cells. MitoTracker Red CMXRos was used to stain mitochondria. Percentage of GFP-positive cells in case of GFP-MTCH2xx was less compared with GFP-MTCH2 and GFP-MTCH2x. One such cell is shown. Scale bar , 10 mum.
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- Figure 4 PHB is bound directly to Mlph in Melan-a cells. A IB analysis for Rab27a, PHB, Mlph, and myosin-Va of Ashen Melan-a cells. B COS7 monkey kidney cells were transfected with PHB and Mlph plasmids. Anti-histidine immunoprecipitated (IP) were performed for 3 h at 4 degC followed by western blot analysis to check co-IP (WB). Whole cell lysates were analyzed for total protein levels.
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- Figure 5. Up-scaling of PEG-precipitation. EVs from 360 mL HEK293T-CD63-eGFP cell conditioned media were prepared by PEG precipitation. The particle concentration per fraction ( n = 3; SD, (a) and their average size distribution ( n = 3; SD, (b) were assessed by NTA (ZetaView). The purity of the obtained samples was determined as particle numbers - measured by NTA - per mg protein content - measured by the BCA assay (c). The presence of the EV marker proteins HSP70, Tsg101, CD63, CD81 and CD9 within the individual PEG samples was analysed by western blot. Additionally, the content of BSA, Syntenin and Prohibitin within the final samples was compared; 5 ug proteins were loaded per lane (d). To test for potential impacts on the physiology of the obtained EVs, uptake experiments were performed: 30-50 ug of the obtained samples were supplemented to the cell culture media of N-KM cells. After 14-16 h pictures were taken, then, the amount of the eGFP labelling was quantified as the mean fluorescence intensity (MFI) by flow cytometry (e). Scale bars 20 um.
