AP15745PU-M

antibody from Acris Antibodies GmbH

Targeting: PHB PHB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP15745PU-M

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP15745PU-M, RRID:AB_10975203

Product name

anti Prohibitin / PHB

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to near C-terminus of Human Prohibitin protein

Reactivity

Human

Host

Rabbit

Vial size

0.5 ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot analysis using Prohibitin / PHB Antibody Cat.-No AP15745PU-S at 2 µg/ml in 1 mg/ml lysate, HeLa 10 µg (Lane 1+2), Raw 20µg (Lane 3+4) lysate, blocking buffer (Blotto; 5% dry milk powder in TBST). R1634HRP anti-Rabbit IgG (H+L)-HRP secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Prohibitin / PHB Antibody Cat.-No AP15745PU-S on Raw 204.4 at 5 µg/ml (Left), incubated with F(ab’)2 Goat anti rabbit IgG (H+L)-FITC conjugated secondary antibody ((R1410F, 1/100). Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human Heart stained with Anti-Prohibitin