10895-1-AP

antibody from Proteintech Group

Targeting: LMNB2 LMN2

Provider product page for 10895-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [35]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 10895-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10895-1-AP, RRID:AB_2136411
Product name: Lamin B2 antibody
Antibody type: Polyclonal
Description: KD/KO validated Lamin B2 antibody (Cat. #10895-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

LMNB2 protein structure - 10895-1-AP shown in red.

Submitted references Stag2-mediated chromatin dynamics regulates antibody class switch recombination.

Wan Z, Yu L, Yang Z, Luo S, Zhou Y, Qiao R, Zha H, Shan X, Wang Y, Li S, Zhang X
Proceedings of the National Academy of Sciences of the United States of America 2026 May 12;123(19):e2536391123

Comparative neurotoxic effects and mechanism of cadmium chloride and cadmium sulfate in neuronal cells.

Mei Z, Yang J, Zhao Y, Li W, Li R, Liu D, Lu H, He Z, Gu S
Environment international 2025 Sep;203:109749

LMNB2-mediated high PD-L1 transcription triggers the immune escape of hepatocellular carcinoma.

Li Y, Zhu J, Zhai F, Ge Y, Zhan Z, Wang S, Kong L, Zhao J, Hu L, Wang S, Shi J, Mao J, Yu Z, Wang H, Jin J, Zhao M, Li H, Jin X
Cell death discovery 2025 Jun 7;11(1):269

MLKL activates the cGAS-STING pathway by releasing mitochondrial DNA upon necroptosis induction.

Ding Z, Wang R, Li Y, Wang X
Molecular cell 2025 Jul 3;85(13):2610-2625.e5

Inhibition of DNA Methyltransferase DNMT1 Reverses Th2 Response Polarisation and Alleviates Allergic Rhinitis.

Zou P, Li J, Li J, Wang J
Clinical and experimental pharmacology & physiology 2025 Feb;52(2):e70015

Exosomes derived from liver failure patients' plasma stimulated mesenchymal stem cells alleviate acute liver failure.

Wang Z, Ling J, You S, Zhu B
Stem cell research & therapy 2025 Feb 7;16(1):48

Consequences of plectin ablation on the various intermediate filament systems in skeletal muscle.

Rocha M, Petsch J, Schultheis D, Zrelski MM, Fichtinger P, Koneczny I, Meier-Menches S, Wiche G, Schröder R, Winter L
European journal of cell biology 2025 Dec;104(4):151522

Oxidative Stress and Annexin A2 Differential Expression in Free Fatty Acids-Induced Non-Alcoholic Fatty Liver Disease in HepG2 Cells.

Arruda VM, Azevedo GT, Granato MJMG, Matos ACP, Araújo TG, Guerra JFDC
International journal of molecular sciences 2024 Sep 4;25(17)

Fructose-1,6-bisphosphatase 1 dephosphorylates and inhibits TERT for tumor suppression.

Li M, Wang Z, Tao J, Jiang H, Yang H, Guo D, Zhao H, He X, Luo S, Jiang X, Yuan L, Xiao L, He H, Yu R, Fang J, Liang T, Mao Z, Xu D, Lu Z
Nature chemical biology 2024 Nov;20(11):1505-1513

C-terminal tagging enhances the detection sensitivity of interlekin receptor type 1.

Moriyama A, Imaoka S, Sasagawa T, Hosaka M, Kato I, Tamura H, Takeuchi R, Tsunoda M, Asano M
Cellular and molecular biology (Noisy-le-Grand, France) 2024 Nov 24;70(10):43-48

UBC9-mediated SUMOylation of Lamin B1 enhances DNA-damage-induced nuclear DNA leakage and autophagy after spinal cord injury.

Fan YP, Lou JS, Jin MR, Zhou CH, Shen HH, Fu CY, Mao XJ, Chen YY, Zhong JJ, Wang LL, Wu JS
Journal of cellular physiology 2024 May;239(5):e31213

The Development of a 3D PET Fibrous Scaffold Modified with an Umbilical Cord dECM for Liver Tissue Engineering.

Li Y, Zhang Y, Zhong K, Liao S, Zhang G
Polymers 2024 Jun 25;16(13)

Forsythoside A mitigates osteoarthritis and inhibits chondrocyte senescence by promoting mitophagy and suppressing NLRP3 inflammasome via the Nrf2 pathway.

Li W, Zhong Y, Lin Z, Deng Z, Long D, Li M, Li C, Mao G, Kang Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Dec;135:156052

YEATS domain-containing protein GAS41 regulates nuclear shape by working in concert with BRD2 and the mediator complex in colorectal cancer.

Wang Z, Zhao N, Zhang S, Wang D, Wang S, Liu N
Pharmacological research 2024 Aug;206:107283

miR-656-3p inhibits melanomas in vitro and in vivo by inducing senescence via inhibiting LMNB2.

Sun J, Lui K, Pang Q, Xu M, Zhao H, Shao J, Yu Y, Chu X, Liang Y, Xu J, Shen Z
Journal of cancer research and clinical oncology 2023 Sep;149(12):10781-10796

Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts.

Zhang T, Zhang Y, Li S, Ge H, Song Q, Zhang Y, Yang G, Li A
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Sep;118:154923

NBDHEX re-sensitizes adriamycin-resistant breast cancer by inhibiting glutathione S-transferase pi.

Sha H, Zou R, Lu Y, Gan Y, Ma R, Feng J, Chen D
Cancer medicine 2023 Mar;12(5):5833-5845

Neutrophil S100A9 supports M2 macrophage niche formation in granulomas.

Mizutani T, Ano T, Yoshioka Y, Mizuta S, Takemoto K, Ouchi Y, Morita D, Kitano S, Miyachi H, Tsuruyama T, Fujiwara N, Sugita M
iScience 2023 Mar 17;26(3):106081

LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion.

Jung-Garcia Y, Maiques O, Monger J, Rodriguez-Hernandez I, Fanshawe B, Domart MC, Renshaw MJ, Marti RM, Matias-Guiu X, Collinson LM, Sanz-Moreno V, Carlton JG
Nature cell biology 2023 Jan;25(1):108-119

Mesenchymal Stem Cells Inhibits Migration and Vasculogenic Mimicry in Nasopharyngeal Carcinoma Via Exosomal MiR-125a.

Wan F, Zhang H, Hu J, Chen L, Geng S, Kong L, Lu JJ
Frontiers in oncology 2022;12:781979

Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.

Wang L, Zhao L, Lin Z, Yu D, Jin M, Zhou P, Ren J, Cheng J, Yang K, Wu G, Zhang T, Zhang D
Clinical and translational medicine 2022 May;12(5):e743

Kruppel like factor 16 promotes lung adenocarcinoma progression by upregulating lamin B2.

Jiao X, Gao W, Ren H, Wu Y, Li T, Li S, Yan H
Bioengineered 2022 Apr;13(4):9482-9494

Dihydrotanshinone I Specifically Inhibits NLRP3 Inflammasome Activation and Protects Against Septic Shock In Vivo.

Wei Z, Zhan X, Ding K, Xu G, Shi W, Ren L, Fang Z, Liu T, Hou X, Zhao J, Li H, Li J, Li Z, Li Q, Lin L, Yang Y, Xiao X, Bai Z, Cao J
Frontiers in pharmacology 2021;12:750815

Antagonizing effects of curcumin against mercury-induced autophagic death and trace elements disorder by regulating PI3K/AKT and Nrf2 pathway in the spleen.

Zhao G, Qi L, Wang Y, Li X, Li Q, Tang X, Wang X, Wu C
Ecotoxicology and environmental safety 2021 Oct 1;222:112529

MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.

Zhang Z, Guo X, Guo X, Yu R, Qian M, Wang S, Gao X, Qiu W, Guo Q, Xu J, Chen Z, Wang H, Qi Y, Zhao R, Xue H, Li G
Aging 2021 Feb 1;13(4):5055-5068

Sestrin2 overexpression alleviates hydrogen peroxide-induced apoptosis and oxidative stress in retinal ganglion cells by enhancing Nrf2 activation via Keap1 downregulation.

Fan Y, Xing Y, Xiong L, Wang J
Chemico-biological interactions 2020 Jun 1;324:109086

Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signalling.

Wang M, Li C, Shi W
Journal of drug targeting 2020 Jul;28(6):600-608

HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway.

Dang X, Zhang R, Peng Z, Qin Y, Sun J, Niu Z, Pei H
Chemico-biological interactions 2020 Jan 25;316:108922

LMNB2 is a prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma.

Kong W, Wu Z, Yang M, Zuo X, Yin G, Chen W
IUBMB life 2020 Dec;72(12):2672-2685

Murine eosinophil development and allergic lung eosinophilia are largely dependent on the signaling adaptor GRB2.

Willebrand R, Dietschmann A, Nitschke L, Krappmann S, Voehringer D
European journal of immunology 2018 Nov;48(11):1786-1795

Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects.

Ge M, Chen C, Yao W, Zhou S, Huang F, Cai J, Hei Z
Oxidative medicine and cellular longevity 2017;2017:8787392

HIV-1 Tat potently stabilises Mdm2 and enhances viral replication.

Raja R, Ronsard L, Lata S, Trivedi S, Banerjea AC
The Biochemical journal 2017 Jul 11;474(14):2449-2464

Lamin B1 and lamin B2 are long-lived proteins with distinct functions in retinal development.

Razafsky D, Ward C, Potter C, Zhu W, Xue Y, Kefalov VJ, Fong LG, Young SG, Hodzic D
Molecular biology of the cell 2016 Jun 15;27(12):1928-37

Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation.

Kuga T, Nie H, Kazami T, Satoh M, Matsushita K, Nomura F, Maeshima K, Nakayama Y, Tomonaga T
Oncogenesis 2014 Mar 17;3(3):e94

Phosphorylation statuses at different residues of lamin B2, B1, and A/C dynamically and independently change throughout the cell cycle.

Kuga T, Nozaki N, Matsushita K, Nomura F, Tomonaga T
Experimental cell research 2010 Aug 15;316(14):2301-12

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 10895-1-AP(LMNB2 antibody) at dilution of 1:2000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using LMNB2 antibody 10895-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 10895-1-AP(LMNB2 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue