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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA5-17274 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Lamin B2 Monoclonal Antibody (GT144)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, HepG2, HepG2 nuclear. Predicted reactivity: Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT144
- Vial size
- 100 µL
- Concentration
- 0.81 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lamin B2 Monoclonal Antibody (GT144) detects LMNB2 protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract. B. 30 µg HepG2 cytosol lysate/extract. C. 30 µg HepG2 nuclear lysate/extract.7.5% SDS-PAGE. Lamin B2 Monoclonal Antibody (GT144) (Product # MA5-17274) dilution: 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lamin B2 Monoclonal Antibody (GT144) detects LMNB2 protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract. B. 30 µg SK-N-SH whole cell lysate/extract. C. 30 µg IMR32 whole cell lysate/extract. D. 30 µg SK-N-AS whole cell lysate/extract.7.5% SDS-PAGE. Lamin B2 Monoclonal Antibody (GT144) (Product # MA5-17274) dilution: 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lamin B2 Monoclonal Antibody (GT144) detects LMNB2 protein at nuclear envelope by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min. Red: LMNB2 protein stained by Lamin B2 Monoclonal Antibody (GT144) (Product # MA5-17274) diluted at 1:1,000. Green: alpha Tubulin antibody (Product # PA5-85157) diluted at 1:1,000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope].
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lamin B2 Monoclonal Antibody (GT144) detects LMNB2 protein at nucleus membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Lamin B2 Monoclonal Antibody (GT144) (Product # MA5-17274) dilution: 1:200. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lamin B2 Monoclonal Antibody (GT144) immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 µg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 µg of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 µg of Lamin B2 Monoclonal Antibody (GT144) (Product # MA5-17274) 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 Monoclonal Antibody (GT144) (Product # MA5-17274) diluted at 1 : 1,000.
