Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX628803 - Provider product page
- Provider
- GeneTex
- Product name
- Lamin B2 antibody [GT144]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Andrographolide suppresses the migratory ability of human glioblastoma multiforme cells by targeting ERK1/2-mediated matrix metalloproteinase-2 expression.
RECQ5 helicase promotes resolution of conflicts between replication and transcription in human cells.
Yang SL, Kuo FH, Chen PN, Hsieh YH, Yu NY, Yang WE, Hsieh MJ, Yang SF
Oncotarget 2017 Dec 1;8(62):105860-105872
Oncotarget 2017 Dec 1;8(62):105860-105872
RECQ5 helicase promotes resolution of conflicts between replication and transcription in human cells.
Urban V, Dobrovolna J, Hühn D, Fryzelkova J, Bartek J, Janscak P
The Journal of cell biology 2016 Aug 15;214(4):401-15
The Journal of cell biology 2016 Aug 15;214(4):401-15
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Enhanced validation
Supportive validation
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- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [GT144] (GTX628803) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
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- Main image
- Experimental details
- Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis.A. 30 ?g U87-MG whole cell lysate/extractB. 30 ?g SK-N-SH whole cell lysate/extract C. 30 ?g IMR32 whole cell lysate/extract D. 30 ?g SK-N-AS whole cell lysate/extract7.5% SDS-PAGELamin B2 antibody [GT144] (GTX628803) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis.A. 30 ?g HepG2 whole cell lysate/extract B. 30 ?g HepG2 cytosol lysate/extractC. 30 ?g HepG2 nuclear lysate/extract7.5% SDS-PAGELamin B2 antibody [GT144] (GTX628803) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [GT144] (GTX628803) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Lamin B2 antibody [GT144] detects LMNB2 protein at nuclear envelope by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min. Red: LMNB2 protein stained by Lamin B2 antibody [GT144] (GTX628803) diluted at 1:1000. Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Lamin B2 antibody [GT144] immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 £gg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 £gg of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 £gg of Lamin B2 antibody [GT144] (GTX628803) 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [GT144] (GTX628803) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Lamin B2 antibody [GT144] detects LMNB2 protein at nucleus membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [GT144] (GTX628803) dilution: 1:200.