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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- RQ7343 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- STK24 Antibody / Serine/threonine-protein kinase 24 / MST-3
- Antibody type
- Polyclonal
- Description
- This highly specific STK24 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the STK24 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat stomach, 2) rat testis, 3) rat brain, 4) mouse stomach, 5) mouse small intestine, 6) mouse testis and 7) mouse brain tissue lysate with STK24 antibody. Predicted molecular weight ~49 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A431, 2) HeLa, 3) Raji, 4) U-87 MG, 5) 293T, 6) placenta, 7) Caco-2 and 8) RT4 cell lysate with STK24 antibody. Predicted molecular weight ~49 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse brain tissue with STK24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain tissue with STK24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-251 cells with STK24 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STK24 antibody.
