PA5-29674

antibody from Invitrogen Antibodies

Targeting: PBX1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29674

Provider

Invitrogen Antibodies

Product name

PBX1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (98%), Chicken (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.7 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PBX1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PBX1 Polyclonal Antibody (Product # PA5-29674) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PBX1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PBX1 Polyclonal Antibody (Product # PA5-29674) at 1:1000 dilution, in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing Nuclear and cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X with Oil immersion magnification.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) assay of endogenous PBX1 protein using PBX1 Antibody: ChIP was performed using Anti-PBX1 Polyclonal Antibody (Product # PA5-29674, 2.5 µg) on sheared chromatin from HeLa cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to SPOCK2 and MEOX1 (Active) and ACTB and SAT2 satellite repeats (Inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PBX1 Polyclonal Antibody immunoprecipitates PBX1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of PBX1 protein by 2 µg of PBX1 Polyclonal Antibody (Product # PA5-29674) 12 % SDS-PAGE The immunoprecipitated PBX1 protein was detected by PBX1 Polyclonal Antibody (Product # PA5-29674) diluted at 1:1,000. .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PBX1 Polyclonal Antibody immunoprecipitates PBX1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of PBX1 protein by 2 µg of PBX1 Polyclonal Antibody (Product # PA5-29674) 12 % SDS-PAGE The immunoprecipitated PBX1 protein was detected by PBX1 Polyclonal Antibody (Product # PA5-29674) diluted at 1:1,000. .