HPA026653

antibody from Atlas Antibodies

Targeting: ERO1A ERO1-alpha, Ero1alpha, ERO1L

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA026653

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA026653, RRID:AB_1848254

Product name

Anti-ERO1A

Antibody type

Polyclonal

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LLQETWLEKKWGHNITEFQQRFDGILTEGEGPRRL
KNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQ
DEENKMLLLEILHEIKSFPLHFDENSFFAGDKKEA
HKLKE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-ERO1A antibody HPA026653 (A) shows similar pattern to independent antibody HPA030053 (B).

Antibody #2 product nr

HPA030053

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human esophagus and skeletal muscle tissues using Anti-ERO1A antibody. Corresponding ERO1A RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, colon, esophagus and testis using Anti-ERO1A antibody HPA026653 (A) shows similar protein distribution across tissues to independent antibody HPA030053 (B).

Antibody #2 product nr

HPA030053

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-ERO1A antibody HPA026653.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-ERO1A antibody HPA026653.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-ERO1A antibody HPA026653.

Sample type

HUMAN