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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA1-46120 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERO1L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This ERO1L antibody is useful for Western Blot, where a band ~57 kDa is observed. Depending on the samples used ERO1L can also appear as a doublet which is believed to represent the oxidized and reduced forms of the protein. Suggested positive control: 293T, INS-1, and MIN6 cell lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis.
Feary M, Racher AJ, Young RJ, Smales CM
Biotechnology progress 2017 Jan;33(1):17-25
Biotechnology progress 2017 Jan;33(1):17-25
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ERO1L in HeLa cells. Samples were incubated in ERO1L polyclonal antibody (Product # PA1-46120).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ERO1L in HeLa cells. Samples were incubated in ERO1L polyclonal antibody (Product # PA1-46120).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Ero1-Lalpha redox shift assay. GS-CHOK1SV cells in suspension were untreated (0 muM BSO) or treated with 20, 100, and 250 muM BSO. In addition reduced (10 mM DTT) and oxidized (10 mM DPS) controls were included. All cell samples were treated with a membrane permeable alkylating agent to freeze redox status following treatment with AMS to determine any change in migration relative to redox controls following SDS and western blot analysis with an antibody for Ero1-Lalpha.
