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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29535 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERO1L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116, MCF-7, mouse stomach. Predicted reactivity: Rat (90%), Pig (96%), Rhesus Monkey (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.95 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERO1L Polyclonal Antibody detects ERO1L protein by Western blot analysis. A. 30 µg HCT116 whole cell lysate/extract. B. 30 µg MCF-7 whole cell lysate/extract.10 % SDS-PAGE. ERO1L Polyclonal Antibody (Product # PA5-29535) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERO1L Polyclonal Antibody detects ERO1L protein by Western blot analysis. A. 50 µg mouse stomach lysate/extract.10 % SDS-PAGE. ERO1L Polyclonal Antibody (Product # PA5-29535) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ERO1L in methanol-fixed Hep3B cells using an ERO1L polyclonal antibody (Product # PA5-29535) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ERO1L (Product # PA5-29535) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
