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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00055234-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055234-M01, RRID:AB_426008
- Product name
- SMU1 monoclonal antibody (M01), clone 2B12-2F2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SMU1.
- Antigen sequence
MSIEIESSDVIRLIMQYLKENSLHRALATLQEETT
VSLNTVDSIESFVADINSGHWDTVLQAIQSLKLPD
KTLIDLYEQVVLELIELRELGAARSLLRQTDPMIM
LKQTQPERYIHLENLLARSYFDPREAYPDGSSKEK
RRAAIAQALAGEVSVVPPSRLMALLGQALKWQQHQ
GLLPPGMTIDLFRGKAAVKDVEEEKFPTQLSRHIK
FGQKSHVECARFSPDGQYLVTGSVDGFIEVWNFTT
GKIRKDLKYQAQDNFMMMDDAVLCMCFSRDTEMLA
TGAQDGKIKVWKIQSGQCLRRFERAHSKGVTCLSF
SKDSSQILSASFDQTIRIHGLKSGKTLKEFRGHSS
FVNEATFTQDGHYIISASSDGTVKIWNMKTTECSN
TFKSLGSTAGTDITVNSVILLPKNPEHFVVCNRSN
TVVIMNMQGQIVRSFSSGKREGGDFVCCALSPRGE
WIYCVGEDFVLYCFSTVTGKLERTLTVHEKDVIGI
AHHPHQNLIATYSEDGLLKLWKP- Isotype
- IgG
- Antibody clone number
- 2B12-2F2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Loss of Smu1 function de-represses DNA replication and over-activates ATR-dependent replication checkpoint.
Ren L, Liu Y, Guo L, Wang H, Ma L, Zeng M, Shao X, Yang C, Tang Y, Wang L, Liu C, Li M
Biochemical and biophysical research communications 2013 Jun 28;436(2):192-8
Biochemical and biophysical research communications 2013 Jun 28;436(2):192-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SMU1 monoclonal antibody (M01), clone 2B12-2F2 Western Blot analysis of SMU1 expression in Hela S3 NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SMU1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
