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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21920 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX6B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, HeLa, HepG2. Predicted reactivity: Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX6B1 using 30 µg of HepG2 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a COX6B1 polyclonal antibody (Product # PA5-21920) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COX6B1 Polyclonal Antibody (Product # PA5-21920). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX6B1 Polyclonal Antibody (Product # PA5-21920) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COX6B1 showing staining in the mitochondria of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a COX6B1 polyclonal antibody (Product # PA5-21920) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COX6B1 Polyclonal Antibody detects COX6B1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COX6B1 protein stained by COX6B1 Polyclonal Antibody (Product # PA5-21920) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BCC3 xenograft, using COX6B1 (Product # PA5-21920) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
