PA5-30644

antibody from Invitrogen Antibodies

Targeting: BRF2 BRFU, FLJ11052, TFIIIB50

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-30644

Provider

Invitrogen Antibodies

Product name

BRF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (88%), Rat (90%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.77 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using BRF2 Polyclonal Antibody (Product # PA5-30644). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: Hela. Lane C: HCT116. 10% SDS PAGE. BRF2 Polyclonal Antibody (Product # PA5-30644) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BRF2 in paraformaldehyde-fixed HeLa cells using a BRF2 polyclonal antibody (Product # PA5-30644) at a 1:200 dilution.