PA5-40128

antibody from Invitrogen Antibodies

Targeting: RPL32 L32

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-40128

Provider

Invitrogen Antibodies

Product name

RPL32 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, HeLa, and HepG2 cells.

Concentration

0.53 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPL32 in various whole cell extracts (30 µg). Samples were separated by 15% SDS-PAGE and the membrane was probed with a RPL32 polyclonal antibody (Product # PA5-40128) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPL32 Polyclonal Antibody detects RPL32 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 Polyclonal Antibody (Product # PA5-40128) diluted at a dilution of 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPL32 Polyclonal Antibody detects RPL32 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL32 protein stained by RPL32 Polyclonal Antibody (Product # PA5-40128) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPL32 Polyclonal Antibody detects RPL32 protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. RPL32 Polyclonal Antibody (Product # PA5-40128) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPL32 Polyclonal Antibody detects RPL32 protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RPL32 Polyclonal Antibody (Product # PA5-40128) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.