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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-40128 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL32 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, HeLa, and HepG2 cells. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (91%), Xenopus laevis (92%), Dog (100%), Pig (100%), Rabbit (94%), Chicken (94%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.53 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- RPL32 Polyclonal Antibody detects RPL32 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL32 protein stained by RPL32 Polyclonal Antibody (Product # PA5-40128) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
