MA5-50492

antibody from Invitrogen Antibodies

Targeting: RPL32 L32

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50492

Provider

Invitrogen Antibodies

Product name

RPL32 Recombinant Rabbit Monoclonal Antibody (HL2331)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat, Canine, Zebrafish

Host

Rabbit

Isotype

IgG

Antibody clone number

HL2331

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) on 293T (30 μg), A431 (30 μg), HeLa (30 μg), and HepG2 (30 μg) whole cell extracts at a dilution of 1:1,000. Extracts were separated by 15% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) in MDCK whole cell extract (30 μg) using a dilution of 1:1,000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) in Whole zebrafish extract (30 μg) using a dilution of 1:1,000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) in Mouse testis and Rat testis samples using a dilution of 1:1,000. Extracts were separated by 15% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) in HeLa cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL32 stained by RPL32 antibody (MA5-50492) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) in Paraffin embedded dog brain and cerebellum tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) in Paraffin embedded mouse brain tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) in Paraffin embedded rat brain tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) on 2 days-post-fertilization zebrafish embryo whole mount at a dilution of 1:100 (Green). Tris-HCl buffer, pH 9.0, 20 min at 70ºC was used for Antigen retrieval.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) on 2 days-post-fertilization zebrafish embryo whole mount at a dilution of 1:100 (Green). Tris-HCl buffer, pH 9.0, 20 min at 70ºC was used for Antigen retrieval.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) in Paraffin embedded rat brain tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPL32 using RPL32 Recombinant Monoclonal Antibody (Product # MA5-50492) on 2 days-post-fertilization zebrafish embryo whole mount at a dilution of 1:100 (Green). Tris-HCl buffer, pH 9.0, 20 min at 70ºC was used for Antigen retrieval.