MA1-80263

antibody from Invitrogen Antibodies

Targeting: S100A9 60B8AG, CAGB, CFAG, CGLB, LIAG, MAC387, MIF, MRP14, NIF, P14

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA1-80263

Provider

Invitrogen Antibodies

Product name

Calprotectin (S100A9) Monoclonal Antibody (MAC387), FITC

Antibody type

Monoclonal

Antigen

Other

Description

This clone recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent studies indicate that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha. The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cellds in a wide range of species. Membrane permeabilization is required for flow cytometry applications. Use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Reactivity

Human, Rat, Bovine, Canine, Feline, Goat, Guinea Pig, Porcine, Rabbit

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

MAC387

Vial size

100 µg

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.