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LearnMAB2065
antibody from R&D Systems
Targeting: S100A9
60B8AG, CAGB, CFAG, CGLB, LIAG, MAC387, MIF, MRP14, NIF, P14
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- MAB2065 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse S100A9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A10, rhS100B, or rhS100P is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P31725- Isotype
- IgG
- Antibody clone number
- 372510
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development.
Interleukin 6 regulates psoriasiform inflammation-associated thrombosis.
The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion.
ID1 promotes breast cancer metastasis by S100A9 regulation.
RAGE expression in tumor-associated macrophages promotes angiogenesis in glioma.
The acute neutrophil response mediated by S100 alarmins during vaginal Candida infections is independent of the Th17-pathway.
Proteomic analysis of the role of S-nitrosoglutathione reductase in lipopolysaccharide-challenged mice.
S100A8/A9 is not involved in host defense against murine urinary tract infection.
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Makthal N, Nguyen K, Do H, Gavagan M, Chandrangsu P, Helmann JD, Olsen RJ, Kumaraswami M
EBioMedicine 2017 Jul;21:131-141
EBioMedicine 2017 Jul;21:131-141
Interleukin 6 regulates psoriasiform inflammation-associated thrombosis.
Wang Y, Golden JB, Fritz Y, Zhang X, Diaconu D, Camhi MI, Gao H, Dawes SM, Xing X, Ganesh SK, Gudjonsson JE, Simon DI, McCormick TS, Ward NL
JCI insight 2016 Dec 8;1(20):e89384
JCI insight 2016 Dec 8;1(20):e89384
The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion.
Dessing MC, Tammaro A, Pulskens WP, Teske GJ, Butter LM, Claessen N, van Eijk M, van der Poll T, Vogl T, Roth J, Florquin S, Leemans JC
Kidney international 2015 Jan;87(1):85-94
Kidney international 2015 Jan;87(1):85-94
ID1 promotes breast cancer metastasis by S100A9 regulation.
Gumireddy K, Li A, Kossenkov AV, Cai KQ, Liu Q, Yan J, Xu H, Showe L, Zhang L, Huang Q
Molecular cancer research : MCR 2014 Sep;12(9):1334-43
Molecular cancer research : MCR 2014 Sep;12(9):1334-43
RAGE expression in tumor-associated macrophages promotes angiogenesis in glioma.
Chen X, Zhang L, Zhang IY, Liang J, Wang H, Ouyang M, Wu S, da Fonseca ACC, Weng L, Yamamoto Y, Yamamoto H, Natarajan R, Badie B
Cancer research 2014 Dec 15;74(24):7285-7297
Cancer research 2014 Dec 15;74(24):7285-7297
The acute neutrophil response mediated by S100 alarmins during vaginal Candida infections is independent of the Th17-pathway.
Yano J, Kolls JK, Happel KI, Wormley F, Wozniak KL, Fidel PL Jr
PloS one 2012;7(9):e46311
PloS one 2012;7(9):e46311
Proteomic analysis of the role of S-nitrosoglutathione reductase in lipopolysaccharide-challenged mice.
Ozawa K, Tsumoto H, Wei W, Tang CH, Komatsubara AT, Kawafune H, Shimizu K, Liu L, Tsujimoto G
Proteomics 2012 Jun;12(12):2024-35
Proteomics 2012 Jun;12(12):2024-35
S100A8/A9 is not involved in host defense against murine urinary tract infection.
Dessing MC, Butter LM, Teske GJ, Claessen N, van der Loos CM, Vogl T, Roth J, van der Poll T, Florquin S, Leemans JC
PloS one 2010 Oct 14;5(10):e13394
PloS one 2010 Oct 14;5(10):e13394
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):7420-7
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):7420-7
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):7420-7
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):7420-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse S100A9 by Simple Western<SUP abp="263">TM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 19 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse S100A9 by Western Blot. Western blot shows lysates of mouse leukocytes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
