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LearnLS-C319039
antibody from LSBio
Targeting: S100A9
60B8AG, CAGB, CFAG, CGLB, LIAG, MAC387, MIF, MRP14, NIF, P14
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C319039 - Provider product page
- Provider
- LSBio
- Product name
- S100A9 / MRP14 Antibody LS-C319039
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: Mouse brain tissue All lanes: S100A9 antibody at 3.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating S100A9 in Mouse spleen tissue Lane 1: Rabbit control IgG instead of S100a9 Antibody in Mouse spleen tissue.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: S100a9 Antibody (8µg) + Mouse spleen tissue (500µg) Lane 3: Mouse spleen tissue (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating S100A9 in Mouse spleen tissue Lane 1: Rabbit control IgG instead of S100a9 Antibody in Mouse spleen tissue.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: S100a9 Antibody (8µg) + Mouse spleen tissue (500µg) Lane 3: Mouse spleen tissue (10µg)
