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LearnV2852
antibody from NSJ Bioreagents
Targeting: S100A9
60B8AG, CAGB, CFAG, CGLB, LIAG, MAC387, MIF, MRP14, NIF, P14
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- V2852 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- S100A9 Antibody
- Antibody type
- Monoclonal
- Antigen
- Human peripheral blood monocyte components were used as the immunogen for the S100A9 antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 47-8D3
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the S100A9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Oxidation of calprotectin by hypochlorous acid prevents chelation of essential metal ions and allows bacterial growth: Relevance to infections in cystic fibrosis.
Fetal human keratinocytes produce large amounts of antimicrobial peptides: involvement of histone-methylation processes.
Global secretome characterization of herpes simplex virus 1-infected human primary macrophages.
The antimicrobial heterodimer S100A8/S100A9 (calprotectin) is upregulated by bacterial flagellin in human epidermal keratinocytes.
Magon NJ, Turner R, Gearry RB, Hampton MB, Sly PD, Kettle AJ
Free radical biology & medicine 2015 Sep;86:133-44
Free radical biology & medicine 2015 Sep;86:133-44
Fetal human keratinocytes produce large amounts of antimicrobial peptides: involvement of histone-methylation processes.
Gschwandtner M, Zhong S, Tschachler A, Mlitz V, Karner S, Elbe-Bürger A, Mildner M
The Journal of investigative dermatology 2014 Aug;134(8):2192-201
The Journal of investigative dermatology 2014 Aug;134(8):2192-201
Global secretome characterization of herpes simplex virus 1-infected human primary macrophages.
Miettinen JJ, Matikainen S, Nyman TA
Journal of virology 2012 Dec;86(23):12770-8
Journal of virology 2012 Dec;86(23):12770-8
The antimicrobial heterodimer S100A8/S100A9 (calprotectin) is upregulated by bacterial flagellin in human epidermal keratinocytes.
Abtin A, Eckhart L, Gläser R, Gmeiner R, Mildner M, Tschachler E
The Journal of investigative dermatology 2010 Oct;130(10):2423-30
The Journal of investigative dermatology 2010 Oct;130(10):2423-30
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human PBM lysate with S100A9 antibody (clone 47-8D3).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free S100A9 Antibody (clone 47-8D3). Confirmation of Integrity and Purity of the Antibody.
