PA5-22272

antibody from Invitrogen Antibodies

Targeting: ASPA ACY2, ASP

Antibody data

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Validation data

Product number: PA5-22272 - Provider product page
Provider: Invitrogen Antibodies
Product name: Aspartoacylase Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: Molt-4, Raji, U87-MG, SK-N-SH, IMR32, SK-N-AS. Predicted reactivity: Mouse (87%), Rat (84%), Pig (95%), Rhesus Monkey (97%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.33 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ASPA protein structure - PA5-22272 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Aspartoacylase using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with an Aspartoacylase polyclonal antibody (Product # PA5-22272) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Aspartoacylase using 30 µg of A) MOLT4 and B) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Aspartoacylase polyclonal antibody (Product # PA5-22272) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Aspartoacylase was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Aspartoacylase Polyclonal Antibody (Product # PA5-22272) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Aspartoacylase Polyclonal Antibody (Product # PA5-22272). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase Polyclonal Antibody (Product # PA5-22272) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Aspartoacylase Polyclonal Antibody (Product # PA5-22272). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Aspartoacylase Polyclonal Antibody (Product # PA5-22272) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.