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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-22272 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aspartoacylase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: Molt-4, Raji, U87-MG, SK-N-SH, IMR32, SK-N-AS. Predicted reactivity: Mouse (87%), Rat (84%), Pig (95%), Rhesus Monkey (97%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1.33 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Aspartoacylase was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Aspartoacylase Polyclonal Antibody (Product # PA5-22272) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Aspartoacylase was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Aspartoacylase Polyclonal Antibody (Product # PA5-22272) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Aspartoacylase (Product # PA5-22272) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
