Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [4]
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Validation data
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- Product number
- MA5-24858 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASH2L Recombinant Rabbit Monoclonal Antibody (2D9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 2D9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASH2L in Lane 1: PC12 whole cell lysate, Lane 2: 293T whole cell lysate. Samples were incubated with ASH2L monoclonal antibody (Product # MA5-24858) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ASH2L in MCF7 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ASH2L monoclonal antibody (Product # MA5-24858).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ASH2L in CRC cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS and incubated with ASH2L monoclonal antibody (Product # MA5-24858).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ASH2L in PC12 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ASH2L monoclonal antibody (Product # MA5-24858).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ASH2L in MCF7 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ASH2L monoclonal antibody (Product # MA5-24858).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASH2L in paraffin-embedded mouse testis tissue. Samples were incubated with ASH2L monoclonal antibody (Product # MA5-24858) followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASH2L in paraffin-embedded mouse placenta tissue. Samples were incubated with ASH2L monoclonal antibody (Product # MA5-24858) followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASH2L in paraffin-embedded mouse colon tissue. Samples were incubated with ASH2L monoclonal antibody (Product # MA5-24858) followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASH2L in paraffin-embedded mouse ovary tissue. Samples were incubated with ASH2L monoclonal antibody (Product # MA5-24858) followed by hematoxylin.