A300-489A

antibody from Invitrogen Antibodies

Targeting: ASH2L ASH2, ASH2L1, ASH2L2, Bre2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-489A

Provider

Invitrogen Antibodies

Product name

ASH2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ASH2 by western blot and immunoprecipitation. Samples: A. Whole cell lysate from 293T cells that were mock transfected (E, 50 µg) or transfected with ASH2 expression constructs containing HA-tagged ASH2 (H, 25 µg) or Flag-tagged ASH2 (F, 25 µg). B. Whole cell lysate from one 10cm plate of normal 293T cells (~1 mg protein; 1/2 of IP loaded/lane). Antibodies: Affinity purified rabbit anti-ASH2 antibody A300-489A used at 1 µg/ml for WB (A and B) and at 5 µg/plate for IP. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody A300-107A using 5 µg/plate. Detection: Chemiluminescence with an exposure time of 1 second (A and B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ASH2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ASH2 (Cat. No. A300-489A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-489A was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.