GTX15668

antibody from GeneTex

Targeting: CD2 SRBC

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

GTX15668

Provider

GeneTex

Product name

CD2 antibody [TS2/18]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of CD2 in 25 ug of Jurkat, CEM and rat thymus lysates. Proteins were transferred to a PVDF membrane and blocked at 4¢XCovernight. The membrane was probed with CD2/LFA-2 antibody [TS2/18] at a dilution of 1:500 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of CD2 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and CD2/LFA-2 antibody [TS2/18] at a dilution of 0.5 ug/test. Cells were incubated for 30 min at 4¢XC and washed with a cell buffer, followed by incubation with a proper secondary antibody. FACS analysis was performed using 400 ul of cell buffer.