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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- UM800017 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800017, RRID:AB_2629130
- Product name
- CD2 mouse monoclonal antibody,clone UMAB86
- Antibody type
- Monoclonal
- Description
- CD2 mouse monoclonal antibody,clone UMAB86
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CD2
- Isotype
- IgG
- Antibody clone number
- UMAB86
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of extracts (35ug) from 10 different cell lines by using anti-CD2 monoclonal antibody (Clone UMAB86)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Jurkat cells using anti-CD2 mouse monoclonal antibody (UM800017, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20?m.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD2 mouse monoclonal antibody. (UM800017; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD2 mouse monoclonal antibody. (UM800017; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human spleen using anti-CD2 mouse monoclonal antibody at 1: 200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800017 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplamic and membrane staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human melanoma using anti-CD2 mouse monoclonal antibody at 1: 200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800017 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplamic and membrane staining infiltrating T cells but no staining in the tumor cells..
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD2 mouse monoclonal antibody (UM800017). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
