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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB6895 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6895, RRID:AB_10677581
- Product name
- CD2 monoclonal antibody, clone UMCD2/IE7E8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against native CD2.
- Isotype
- IgG
- Antibody clone number
- UMCD2/IE7E8
- Storage
- Store at 4°C.
Submitted references CD2 and the regulation of T cell anergy.
An anti-CD2 monoclonal antibody that both inhibits and stimulates T cell activation recognizes a subregion of CD2 distinct from known ligand-binding sites.
Bell GM, Imboden JB
Journal of immunology (Baltimore, Md. : 1950) 1995 Sep 15;155(6):2805-7
Journal of immunology (Baltimore, Md. : 1950) 1995 Sep 15;155(6):2805-7
An anti-CD2 monoclonal antibody that both inhibits and stimulates T cell activation recognizes a subregion of CD2 distinct from known ligand-binding sites.
Kozarsky KF, Tsai C, Bott CM, Allada G, Li LL, Fox DA
Cellular immunology 1993 Sep;150(2):235-46
Cellular immunology 1993 Sep;150(2):235-46
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- 5 X 105 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80 uL of CD2 monoclonal antibody, clone UMCD2/IE7E8 (Cat # MAB6895) at 1 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.12 log 10 fluorescent units when compared to a Mouse IgG2a negative control.
- Validation comment
- Flow Cytometry
