TA500404

antibody from OriGene

Targeting: CD2 SRBC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500404

Provider

OriGene

Proper citation

OriGene Cat#TA500404, RRID:AB_2073546

Product name

Anti-CD2 mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)

Antibody type

Monoclonal

Description

Anti-CD2 mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)

Host

Mouse

Conjugate

Unconjugated

Epitope

CD2

Isotype

IgG

Antibody clone number

OTI4E4

Vial size

100 µl

Concentration

0.6 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD2 (RC206612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of live Jurkat cells, using anti-CD2 antibody (TA500404 at 1:100, Red), compared to IgG1 isotype control (Black).

Validation comment

FC