Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 64-0029-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD2 Monoclonal Antibody (RPA-2.10), Super Bright™ 645, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.
- Antibody clone number
- RPA-2.10
- Concentration
- 5 µL/Test
Submitted references Constraints on GPCR Heterodimerization Revealed by the Type-4 Induced-Association BRET Assay.
Felce JH, MacRae A, Davis SJ
Biophysical journal 2019 Jan 8;116(1):31-41
Biophysical journal 2019 Jan 8;116(1):31-41
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and Mouse IgG1 kappa Isotype Control, Super Bright 645 (Product # 64-4714-82) (left) or CD2 Monoclonal Antibody, Super Bright 645 (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Type-4 BRET assay applied to known controls. ( A ) The mean Delta BRET eff of type-1 transmembrane protein controls using the FKBP 1 type-4 BRET assay. Only the known dimers CD28 and CD80 exhibited significantly nonzero Delta BRET eff when expressed as homomeric pairs. Dark gray bars indicate like-like pairs. ( B ) The mean Delta BRET eff of type-1 transmembrane protein controls using the FKBP 3 type-4 BRET assay. Delta BRET eff is larger for CD28 and CD80 than with the FKBP 1 approach. ( C ) A histogram of CD2 expression in HEK-293T cells transfected with 1 mu g of differently tagged forms of CD2, as measured by flow cytometry. ( D ) Confocal microscopy of CD28-GFP coexpressed with CD28-FKBP 1/3 in the presence and absence of AP20187. No clear GFP internalization was evident. ( E ) The mean Delta BRET eff of GPCRs of known stoichiometry using the FKBP 3 type-4 BRET assay. For all panels, bars indicate mean +- SD. Probability is indicated for difference from Delta BRET eff = 0; * p < 0.05, *** p < 0.005. All data are the result of n >= 3 independent experiments.