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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C800178 - Provider product page
- Provider
- LSBio
- Product name
- CD2 Antibody (clone UMAB6) LS-C800178
- Antibody type
- Monoclonal
- Description
- Affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB6
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY CD2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2. (1:500)
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts. (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody. (Clone UMAB6). (1:1000)
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human spleen using anti-CD2 clone UMAB6 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Citrate pH6.O. The image shows strong membranous and cytoplasmic staining.
