UM500006CF

antibody from Invitrogen Antibodies

Targeting: CD2 SRBC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

UM500006CF

Provider

Invitrogen Antibodies

Product name

CD2 Monoclonal Antibody (UMAB6), UltraMAB™

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

UMAB6

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD2(RC206612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2. (1:500)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-CD2 monoclonal antibody (Clone UMAB6). (1:1000)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Jurkat cells using anti-CD2 mouse monoclonal antibody (UM500006, green, 1:100). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 8µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IHC staining of paraffin-embedded human tonsil using anti-CD2 clone UMAB6 mouse monoclonal antibody at 1:200 of 0.6mg/mL and detection with Polink2 Broad HRP DAB. UM500006 requires heat-induced epitope retrieval with Accel pH8.7 at 95-100C 30 minutes [do not let boil] or 10 min in pressure cooker. The image shows strong membranous and cytoplasmic staining in >50 % of germinal center cells of tonsil and

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IHC staining of FFPE human spleen using anti-CD2 clone UMAB6 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB. UM500006 requires heat-induced epitope retrieval with Citrate pH6.O. The image shows strong membranous and cytoplasmic staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-CD2 mouse monoclonal antibody. (Clone UMAB6, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CD2 mouse monoclonal antibody. (Clone UMAB6, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue anti-CD2 clone UMAB6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM500006)(1:500)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded mouse colon tissue within the normal limits using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM500006)(1:500)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD2 clone UMAB6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM500006)(1:500).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of living Jurkat cells, using anti-CD2 antibody (UM500006, Red), compared to an isotype control (green), and a PBS control (blue). (1:100)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD2 mouse monoclonal antibody (Clone UMAB6). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-CD2 (Clone UMAB6) very specifically recognizes Cd2 antigen on OriGene protein microarray chip.