MA5-11373

antibody from Invitrogen Antibodies

Targeting: CD2 SRBC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-11373

Provider

Invitrogen Antibodies

Product name

CD2 Monoclonal Antibody (AB75)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

MA5-11373 targets CD2 in IHC (P) and FACS applications and shows reactivity with Human samples. The MA5-11373 immunogen is recombinant fragment encoding the external domain of the human CD2 protein.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

AB75

Vial size

500 μL

Concentration

Conc. Not Determined

Storage

4°C

References

Submitted references

Nontuberculous mycobacterial infection with concurrent IgG4-related lymphadenopathy.

Liu TT, Weng SW, Wang MC, Huang WT
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2016 Mar;124(3):216-20

---

Infectious mononucleosis mimicking malignant T-cell lymphoma in the nasopharynx: a case report and review of the literature.

He HL, Wang MC, Huang WT
International journal of clinical and experimental pathology 2013;6(1):105-9

---

Human Immunodeficiency Virus (HIV)-Negative and Human Herpes Virus-8 (HHV-8)-Positive Primary Effusion Lymphoma: A Case Report and Review of the Literature.

Güven Karataş S, Bayrak R, Sahin Balçık O, Yalçın KS, Atıcı E, Akyıldız U, Koşar A
Turkish journal of haematology : official journal of Turkish Society of Haematology 2013 Mar;30(1):67-71

---

Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases.

Chang IW, Chen HK, Ma MC, Huang WT
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2011 Nov;119(11):794-801

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Ramos (Lane1), A-431 (Lane2), K562 (Lane 3) and Raji (Lane4). The blots were probed with Anti-CD2 Mouse Monoclonal Antibody (Product # MA0200, 1:100-1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 40 kDa band corresponding to CD2 was observed across cell lines tested expect for K562. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0341BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with CD2 antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of T lymphocytes.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD2 monoclonal antibody (Product # MA5-11373) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD2 monoclonal antibody (Product # MA5-11373) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.