HPA003883

CD2 antibody from Atlas Antibodies

SRBC

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunohistochemistry

Enhanced validation

Provider product page for HPA003883

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Antibody Data

Product number

HPA003883

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003883, RRID:AB_1233734

Product name

Anti-CD2

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DEELETRAHRVATEERGRKPHQIPASTPQNPATSQ
HPPPPPGHRSQAPSHRPPPPGHRVQHQPQKRPPAP
SGTQVHQQKGPPLPRPRVQPKPPHGAAENSLSP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

CD73-generated extracellular adenosine in chronic lymphocytic leukemia creates local conditions counteracting drug-induced cell death
Serra S, Horenstein A, Vaisitti T, Brusa D, Rossi D, Laurenti L, D'Arena G, Coscia M, Tripodo C, Inghirami G, Robson S, Gaidano G, Malavasi F, Deaglio S
Blood 2011 December;118(23):6141-6152

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and CD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400671).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human tonsil and cerebral cortex tissues using Anti-CD2 antibody. Corresponding CD2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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