AI10147

antibody from Abgent

Targeting: MBD4 MED1

Western blot (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

AI10147

Provider

Abgent

Product name

MBD4 antibody - middle region

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Vial size

50 µg

Storage

"Add 50 ul of distilled water. Final anti-MBD4 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles."

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"MBD4 antibody - middle region (AI10147) in Human HeLa cells using Western BlotWB Suggested Anti-MBD4 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Positive Control: Hela cell lysateMBD4 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells"

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"MBD4 antibody - middle region (AI10147) in HCT116 using CHIPQuiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site."