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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347242 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal MBD4 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal MBD4 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MBD4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MBD4(lane 2), diluted 1:2,000 in TBST containing 3% milk powder. Figure 2B: WB was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 ug) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human MBD4 in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.
- Validation comment
- ELISA
