Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-51670 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MBD4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LQNQSNNSNW NLRTRSKCKK DVFMPPSSSS ELQESRGLSN FTSTHLLLKE DEGVDDVNFR KVRKPKGKVT ILKGIPIKKT KKGCRKSCSG FVQSDSKRES VCNKADAESE PVAQKSQLDR TVCISDAGAC GETLSVTSEE N
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MBD4 in Lane 1: Marker (kDa) 230, 110, 82, 49, 32, 26, 18; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human cell line A-431; Lane 5: Human liver tissue. Samples were probed using a MBD4 Polyclonal Antibody (Product # PA5-51670).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MBD4 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a MBD4 Polyclonal Antibody (Product # PA5-51670).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MBD4 in human breast cancer using MBD4 Polyclonal Antibody (Product # PA5-51670) shows moderate to strong nuclear positivity in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MBD4 in human cerebellum using MBD4 Polyclonal Antibody (Product # PA5-51670) shows moderate to strong nuclear speckle positivity in Purkinje cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MBD4 in human lymph node using MBD4 Polyclonal Antibody (Product # PA5-51670) shows moderate to strong nuclear positivity in germinal center cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MBD4 in human skin using MBD4 Polyclonal Antibody (Product # PA5-51670) shows moderate to strong nuclear speckle positivity in squamous epithelial cells.