H006T03Y07

antibody from Invitrogen Antibodies

Targeting: FCGR3B CD16, CD16b, FCG3, FCGR3

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

H006T03Y07

Provider

Invitrogen Antibodies

Product name

CD16 Monoclonal Antibody (3G8), NovaFluor™ Yellow 730, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This 3G8 monoclonal antibody reacts with human and non-human primate CD16, which is also known as the low-affinity Fc gamma RIII. CD16 exists as two distinct isoforms, Fc gamma RIIIA and Fc gamma RIIIB. In humans, Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor. Fc gamma RIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in non-human primates. However, while CD16 is not found on neutrophils in macaques and baboons, this receptor is detected on these cells in sooty mangabeys. Binding of IgG leads to activation of signal transduction pathways, resulting in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation.

Antibody clone number

3G8

Concentration

4 µL/Test

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Spectral signature for NovaFluor Yellow 730 collected on a 5-laser Cytek Aurora Full Spectrum flow cytometer using Cytek assay settings. Human peripheral blood mononuclear cells were stained with anti-human CD4 (SK3) and signatures displayed following gating on the lymphocyte population.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human PBMCs with side scatter and unstained (left) or CD16 Monoclonal Antibody, NovaFluor Yellow 730 (right). Data was acquired in the YG8 channel on a 5-laser Cytek Aurora and singlet cells were used for analysis.