PA5-22017

antibody from Invitrogen Antibodies

Targeting: DNASE1 DNL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22017

Provider

Invitrogen Antibodies

Product name

DNase I Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Molt-4. Predicted reactivity: Dog (81%), Rabbit (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.19 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Role of Bacterial and Host DNases on Host-Pathogen Interaction during Streptococcus suis Meningitis.

Meurer M, Öhlmann S, Bonilla MC, Valentin-Weigand P, Beineke A, Hennig-Pauka I, Schwerk C, Schroten H, Baums CG, Köckritz-Blickwede MV, de Buhr N
International journal of molecular sciences 2020 Jul 25;21(15)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DNase I using 30 µg of MOLT4 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a DNase I polyclonal antibody (Product # PA5-22017) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DNase I was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DNase I Polyclonal Antibody (Product # PA5-22017) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of DNase I was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: DNase I Polyclonal Antibody (Product # PA5 22017) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DNase I Polyclonal Antibody detects DNase I protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. DNase I Polyclonal Antibody (Product # PA5-22017) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DNase I Polyclonal Antibody detects DNase I protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. DNase I Polyclonal Antibody (Product # PA5-22017) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Host DNases are detectable and active during S. suis meningitis in choroid plexus, CSF and serum. ( A ) By immunofluorescence staining, we could show that the amount of DNase 1 in the choroid plexus is different in animals with inflammation, compared to animals without inflammation of the choroid plexus. In the choroid plexus of an infected pig without clinical signs of CNS disorders only weak DNase 1 signals were detected in the early phase experiment. In pigs with clinical signs of CNS disorders a high DNase 1 signal in the region of the choroid plexus epithelial cells was observed. PR-39 signal, as a marker for neutrophils, was found in these pigs as well (blue = DNA (Hoechst); green = PR-39; red = DNase 1 (scale bar upper panel = 50 um; lower panel = 20 um; arrows mark transmigrating or already transmigrated PR-39 positive neutrophils). Activity of DNases was observed in serum ( B ) and in CSF ( C ) of all pigs of the early phase experiment. A significant increase of DNase activity post-infection was found in serum for both infection groups after 48 h and for the S. suis 10-infected group also after 96 h. In CSF after 96 h, a higher increase was observed than after 48 h in both infection groups. Data shown as mean +- SD. Statistical analysis: unpaired Mann-Whitney test (* p < 0.05, ** p < 0.01).