Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-27175 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR146 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references RNA-Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts.
Azar C, Valentine MC, Trausch-Azar J, Rois L, Mahjoub M, Nelson DM, Schwartz AL
Physiological reports 2021 Jan;9(1):e14671
Physiological reports 2021 Jan;9(1):e14671
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPR146 using 30 µg of H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GPR146 polyclonal antibody (Product # PA5-27175) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GPR146 Polyclonal Antibody (Product # PA5-27175). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPR146 Polyclonal Antibody (Product # PA5-27175) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.