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LearnGTX107748
antibody from GeneTex
Targeting: GPER1
CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR
Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- GTX107748 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107748, RRID:AB_1240855
- Product name
- GPR30 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Estrogen receptor signaling in the ferutinin-induced osteoblastic differentiation of human amniotic fluid stem cells.
Estradiol rapidly modulates odor responses in mouse vomeronasal sensory neurons.
Estradiol and tamoxifen induce cell migration through GPR30 and activation of focal adhesion kinase (FAK) in endometrial cancers with low or without nuclear estrogen receptor α (ERα).
Zavatti M, Guida M, Maraldi T, Beretti F, Bertoni L, La Sala GB, De Pol A
Life sciences 2016 Nov 1;164:15-22
Life sciences 2016 Nov 1;164:15-22
Estradiol rapidly modulates odor responses in mouse vomeronasal sensory neurons.
Cherian S, Wai Lam Y, McDaniels I, Struziak M, Delay RJ
Neuroscience 2014 Jun 6;269:43-58
Neuroscience 2014 Jun 6;269:43-58
Estradiol and tamoxifen induce cell migration through GPR30 and activation of focal adhesion kinase (FAK) in endometrial cancers with low or without nuclear estrogen receptor α (ERα).
Tsai CL, Wu HM, Lin CY, Lin YJ, Chao A, Wang TH, Hsueh S, Lai CH, Wang HS
PloS one 2013;8(9):e72999
PloS one 2013;8(9):e72999
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (40ug of whole cell lysate) A: RL952 cell B: GPR30 shRNA-transfected RL952 cell GTX107748 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: H1299 B: HeLa 10% SDS PAGE GTX107748 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GPR30 antibody [C2C3], C-term detects GPR30 protein by western blot analysis.A. 50 ?g 293T whole cell lysate/extract B. 50 ?g whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling) C. 50 ?g whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling)5% SDS-PAGEGPR30 antibody [C2C3], C-term (GTX107748) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) unboiled 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(GTX107748) antibody at 1:150 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(GTX107748) antibody at 1:150 dilution.
