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LearnPA5-87717
antibody from Invitrogen Antibodies
Targeting: GPER1
CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [5]
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- Product number
- PA5-87717 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR30 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HIVNLAAFSN SCLNPLIYSF LGETFRDKLR LYIEQKTNLP ALNRFCHAAL KAVIPDSTEQ SDVRFSSAV
- Concentration
- 2.41 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using GPER1 Polyclonal antibody (Product # PA5-87717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPR30 in extracts of various cell lines using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of C6 cells using GPER1 Polyclonal antibody (Product # PA5-87717) at dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GPR30 was performed in NIH/3T3 cells using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GPR30 was performed in C6 cells using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GPR30 was performed in NIH/3T3 cells using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GPR30 was performed in U2OS cells using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:100. Blue: DAPI for nuclear staining.
