PA5-87717

antibody from Invitrogen Antibodies

Targeting: GPER1 CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87717

Provider

Invitrogen Antibodies

Product name

GPR30 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: HIVNLAAFSN SCLNPLIYSF LGETFRDKLR LYIEQKTNLP ALNRFCHAAL KAVIPDSTEQ SDVRFSSAV Positive Samples: Mouse brain, Mouse heart, Rat brain; Cellular Location: Basolateral cell membrane, Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle membrane, Early endosome, Endoplasmic reticulum membrane, Golgi apparatus, Golgi apparatus membrane, Mitochondrion membrane, Multi-pass membrane protein, Nucleus, Recycling endosome, axon, cytoskeleton, dendrite, dendritic spine membrane, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse, trans-Golgi network

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.66 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using GPER1 Polyclonal antibody (Product # PA5-87717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GPR30 in extracts of various cell lines using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of C6 cells using GPER1 Polyclonal antibody (Product # PA5-87717) at dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GPR30 was performed in NIH/3T3 cells using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GPR30 was performed in C6 cells using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GPR30 was performed in NIH/3T3 cells using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GPR30 was performed in U2OS cells using GPR30 Polyclonal Antibody (Product # PA5-87717) at a dilution of 1:100. Blue: DAPI for nuclear staining.