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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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- Product number
- NBP2-61224 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal CARKL Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store the unopened product at 2 - 8degrees C. Protect from light. Do not use past expiration date.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: CARKL Antibody [NBP2-61224] - Detection of Human SHPK by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHPK antibody NBP2-61224 used for IP at 20 ul per reaction. SHPK was also immunoprecipitated by rabbit anti-SHPK antibody NBP2-61223. For blotting immunoprecipitated SHPK, NBP2-61224 was used at 1:100. Detection: Chemiluminescence with an exposure time of 3 minutes.
