TA503209

antibody from OriGene

Targeting: SHPK CARKL, SHK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503209

Provider

OriGene

Proper citation

OriGene Cat#TA503209, RRID:AB_11126441

Product name

SHPK (CARKL) mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)

Antibody type

Monoclonal

Description

SHPK (CARKL) mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)

Host

Mouse

Conjugate

Unconjugated

Epitope

SHPK

Isotype

IgG

Antibody clone number

OTI1D2

Vial size

100 µl

Concentration

0.63 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SHPK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPK (RC204421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPK.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-SHPK mouse monoclonal antibody (TA503209) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHPK(RC204421).

Validation comment

IF