HPA049032

antibody from Atlas Antibodies

Targeting: ITCH AIP4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA049032

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA049032, RRID:AB_2680607

Product name

Anti-ITCH

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

GFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGE
NRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRR
PASVNGS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines Caco-2 and HEK293 using Anti-ITCH antibody. Corresponding ITCH RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

Sample type

HUMAN