Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- MAB10272-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EBI2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human EBI2 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P32249
- Isotype
- IgG
- Antibody clone number
- 486633
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of EBI2 in HEK293 Human Cell Line Transfected with Human EBI2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) EBI2 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human EBI2 Monoclonal Antibody (Catalog # MAB10272) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2A Isotype Control Antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of EBI2 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human EBI2 Monoclonal Antibody (Catalog # MAB10272, filled histogram) or Mouse IgG2A Isotype Control Antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.