Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- HPA003340 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA003340, RRID:AB_1856531
- Product name
- Anti-S100A10
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human S100A10, Gene description: S100 calcium binding protein A10, Alternative Gene Names: 42C, ANX2LG, CAL1L, CLP11, P11, Validated applications: ICC, IHC, WB, Uniprot ID: P60903, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references A High-throughput Bead-based Affinity Assay Enables Analysis of Genital Protein Signatures in Women At Risk of HIV Infection
CPT1A‐mediated succinylation of S100A10 increases human gastric cancer invasion
Månberg A, Bradley F, Qundos U, Guthrie B, Birse K, Noël-Romas L, Lindskog C, Bosire R, Kiarie J, Farquhar C, Burgener A, Nilsson P, Broliden K
Molecular & Cellular Proteomics 2019;18(3):461-476
Molecular & Cellular Proteomics 2019;18(3):461-476
CPT1A‐mediated succinylation of S100A10 increases human gastric cancer invasion
Wang C, Zhang C, Li X, Shen J, Xu Y, Shi H, Mu X, Pan J, Zhao T, Li M, Geng B, Xu C, Wen H, You Q
Journal of Cellular and Molecular Medicine 2018;23(1):293-305
Journal of Cellular and Molecular Medicine 2018;23(1):293-305
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Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting S100A10 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting S100A10 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:94
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human lung and skeletal muscle tissues using HPA003340 antibody. Corresponding S100A10 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol