LS-C291050

antibody from LSBio

Targeting: P2RX5 LRH-1, P2X5

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C291050

Provider

LSBio

Product name

Anti-P2RX5 / P2X5 Antibody (aa372-422) LS-C291050

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human P2RX5 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-P2RX5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human P2RX5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-P2RX5 antibody used for IP at 6 ug per reaction. P2RX5 was also immunoprecipitated by rabbit anti-P2RX5 antibody BL16575. For blotting immunoprecipitated P2RX5, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.