PA5-31863

antibody from Invitrogen Antibodies

Targeting: TFF1 BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31863

Provider

Invitrogen Antibodies

Product name

TFF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: MCF-7. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.17 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

TFF-1 Functions to Suppress Multiple Phenotypes Associated with Lung Cancer Progression.

Minegishi K, Dobashi Y, Tsubochi H, Hagiwara K, Ishibashi Y, Nomura S, Nakamura R, Ohmoto Y, Endo S
OncoTargets and therapy 2021;14:4761-4777

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Heparanase Promotes Syndecan-1 Expression to Mediate Fibrillar Collagen and Mammographic Density in Human Breast Tissue Cultured ex vivo.

Huang X, Reye G, Momot KI, Blick T, Lloyd T, Tilley WD, Hickey TE, Snell CE, Okolicsanyi RK, Haupt LM, Ferro V, Thompson EW, Hugo HJ
Frontiers in cell and developmental biology 2020;8:599

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Trefoil factor 1 in methanol-fixed MCF-7 cells using a Trefoil factor 1 polyclonal antibody (Product # PA5-31863) at a 1:500 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Expression of endogenous TFF-1 protein in cultured cells from human lung carcinoma. ( A ) Expression of the TFF-1 protein was detected at around 13kDa by immunoblotting analysis only in PC-3 cells. ( B ) Schematic presentation of TFF-1 expression and the amount secreted into the medium analyzed by ELISA. ( C ) Result of qRT-PCR analysis of TFF-1 mRNA expression, expressed relative to values obtained for H1975. ( B and C ) Square and bar indicate mean value and standard deviation, respectively, obtained from three replicated experiments with three replicate samples. Abbreviations : AC, adenocarcinoma; SCC, squamous cell carcinoma; LCC, large cell carcinoma; SmCC, small cell carcinoma; Marker, molecular weight marker.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Establishment of TFF-1-overexpressing cell lines. SBC-5 and LC-AI cells were transfected with a vector containing the cDNA encoding TFF-1/FLAG (pBA-TFF1) or empty vector (pBA-neo). ( A ) Expression of this fusion protein was evaluated by immunoblotting analysis with anti-TFF-1 antibody. Expression was not observed in parental SBC-5 or LC-AI cells (BC), but a single band of around 15kDa was detected after transfection of pBA-TFF1 (G-TFF1) in 4 clones, but not observed in cells transfected with pBA-neo (G-neo). Expression was normalized to the beta-actin loading control. ""Marker"" indicates molecular weight marker. ( B and C ) Images of immunofluorescent staining in parental cells (BC), pBA-neo (G-neo) or pBA-TFF1-transfected cells (G-TFF1) of SBC-5 ( B ), LC-AI ( C ) cells are shown. Cells were stained with anti-TFF-1 antibody and with 4',6-diamidino-2-phenylindole (DAPI). Scale bar indicates 100mum. ( D ) Schematic presentation of TFF-1 mRNA expression after transfection with pBA-neo or pBA-TFF1 (3 clones; CL) expressed relative to values obtained for H1975. Square and bar indicate mean values and standard deviations, respectively, obtained by qRT-PCR analysis from three replicated experiments with three replicate samples. Results were statistically analyzed and p-values were obtained by Fisher's PLSD test.