Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA003425 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA003425, RRID:AB_1848260
- Product name
- Anti-TFF1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human TFF1, Gene description: trefoil factor 1, Alternative Gene Names: BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2, Validated applications: IHC, WB, Uniprot ID: P04155, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.4 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Downregulation of the Tumor Suppressor TFF1 Is Required during Induction of Colon Cancer Progression by L1
A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression
In situ protein detection with enhanced specificity using DNA-conjugated antibodies and proximity ligation
Dynamics of Protein Expression Reveals Primary Targets and Secondary Messengers of Estrogen Receptor Alpha Signaling in MCF-7 Breast Cancer Cells
Antibody-based Protein Profiling of the Human Chromosome 21
Gene expression signatures differentiate ovarian/peritoneal serous carcinoma from breast carcinoma in effusions
Candidate Serological Biomarkers for Cancer Identified from the Secretomes of 23 Cancer Cell Lines and the Human Protein Atlas
Gene expression signatures differentiate ovarian/peritoneal serous carcinoma from breast carcinoma in effusions
The Human Protein Atlas—a tool for pathology
Saha A, Gavert N, Brabletz T, Ben-Ze’ev A
Cancers 2022;14(18):4478
Cancers 2022;14(18):4478
A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression
Liu J, Ottaviani D, Sefta M, Desbrousses C, Chapeaublanc E, Aschero R, Sirab N, Lubieniecki F, Lamas G, Tonon L, Dehainault C, Hua C, Fréneaux P, Reichman S, Karboul N, Biton A, Mirabal-Ortega L, Larcher M, Brulard C, Arrufat S, Nicolas A, Elarouci N, Popova T, Némati F, Decaudin D, Gentien D, Baulande S, Mariani O, Dufour F, Guibert S, Vallot C, Rouic L, Matet A, Desjardins L, Pascual-Pasto G, Suñol M, Catala-Mora J, Llano G, Couturier J, Barillot E, Schaiquevich P, Gauthier-Villars M, Stoppa-Lyonnet D, Golmard L, Houdayer C, Brisse H, Bernard-Pierrot I, Letouzé E, Viari A, Saule S, Sastre-Garau X, Doz F, Carcaboso A, Cassoux N, Pouponnot C, Goureau O, Chantada G, de Reyniès A, Aerts I, Radvanyi F
Nature Communications 2021;12(1)
Nature Communications 2021;12(1)
In situ protein detection with enhanced specificity using DNA-conjugated antibodies and proximity ligation
Zieba A, Ponten F, Uhlén M, Landegren U
Modern Pathology 2018;31(2):253-263
Modern Pathology 2018;31(2):253-263
Dynamics of Protein Expression Reveals Primary Targets and Secondary Messengers of Estrogen Receptor Alpha Signaling in MCF-7 Breast Cancer Cells
Drabovich A, Pavlou M, Schiza C, Diamandis E
Molecular & Cellular Proteomics 2016;15(6):2093-2107
Molecular & Cellular Proteomics 2016;15(6):2093-2107
Antibody-based Protein Profiling of the Human Chromosome 21
Uhlén M, Oksvold P, Älgenäs C, Hamsten C, Fagerberg L, Klevebring D, Lundberg E, Odeberg J, Pontén F, Kondo T, Sivertsson Å
Molecular & Cellular Proteomics 2012;11(3):M111.013458
Molecular & Cellular Proteomics 2012;11(3):M111.013458
Gene expression signatures differentiate ovarian/peritoneal serous carcinoma from breast carcinoma in effusions
Davidson B, Stavnes H, Holth A, Chen X, Yang Y, Shih I, Wang T
Journal of Cellular and Molecular Medicine 2011;15(3):535-544
Journal of Cellular and Molecular Medicine 2011;15(3):535-544
Candidate Serological Biomarkers for Cancer Identified from the Secretomes of 23 Cancer Cell Lines and the Human Protein Atlas
Wu C, Hsu C, Chen C, Yu C, Chang K, Tai D, Liu H, Su W, Chang Y, Yu J
Molecular & Cellular Proteomics 2010;9(6):1100-1117
Molecular & Cellular Proteomics 2010;9(6):1100-1117
Gene expression signatures differentiate ovarian/peritoneal serous carcinoma from breast carcinoma in effusions
Davidson B, Stavnes H, Holth A, Chen X, Yang Y, Shih I, Wang T
Journal of Cellular and Molecular Medicine 2010 January
Journal of Cellular and Molecular Medicine 2010 January
The Human Protein Atlas—a tool for pathology
Pontén F, Jirström K, Uhlen M
The Journal of Pathology 2008;216(4):387-393
The Journal of Pathology 2008;216(4):387-393
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and TFF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418822).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human stomach and liver tissues using Anti-TFF1 antibody. Corresponding TFF1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol